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XPD antibody

Anti-XPD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108948
Anti-XPD antibody used in IHC (Paraffin sections) (IHC-P). GTX108948
Anti-XPD antibody used in Western Blot (WB). GTX108948

Cat No. GTX108948

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human XPD. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10727593

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ERCC excision repair 2, TFIIH core complex helicase subunit , COFS2 , EM9 , TFIIH , TTD , TTD1 , XPD

Cellular Localization

Nucleus. Cytoplasm ? cytoskeleton ? spindle

Background

The nucleotide excision repair pathway is a mechanism to repair damage to DNA. The protein encoded by this gene is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. The gene product has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in this gene can result in three different disorders, the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-XPD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108948

GTX108948 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using ERCC2(GTX108948) antibody at 1:500 dilution.

Anti-XPD antibody used in IHC (Paraffin sections) (IHC-P). GTX108948

GTX108948 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung cancer, using ERCC2(GTX108948) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-XPD antibody used in Western Blot (WB). GTX108948

GTX108948 WB Image

ERCC2 antibody detects ERCC2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ERCC2 antibody (GTX108948) diluted at 1:1000.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169