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XPD antibody

Anti-XPD antibody used in Western Blot (WB). GTX108948
Anti-XPD antibody used in IHC (Paraffin sections) (IHC-P). GTX108948
Anti-XPD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108948

Cat. No. GTX108948

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all XPD products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human XPD. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10727593

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ERCC excision repair 2, TFIIH core complex helicase subunit , COFS2 , EM9 , TFIIH , TTD , TTD1 , XPD

Cellular Localization

Nucleus. Cytoplasm ? cytoskeleton ? spindle

Background

The nucleotide excision repair pathway is a mechanism to repair damage to DNA. The protein encoded by this gene is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. The gene product has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in this gene can result in three different disorders, the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-XPD antibody used in Western Blot (WB). GTX108948

GTX108948 WB Image

ERCC2 antibody detects ERCC2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ERCC2 antibody (GTX108948) diluted at 1:1000.

Anti-XPD antibody used in IHC (Paraffin sections) (IHC-P). GTX108948

GTX108948 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung cancer, using ERCC2(GTX108948) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-XPD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108948

GTX108948 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using ERCC2(GTX108948) antibody at 1:500 dilution.

REFERENCE

Application Reference

Tan HW et al. Cells 2019; 8 (8) Lasting DNA Damage and Aberrant DNA Repair Gene Expression Profile Are Associated with Post-Chronic Cadmium Exposure in Human Bronchial Epithelial Cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169