APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1-0.3μg/ml |
| 5μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88401-PEP).
Product Note
This antibody is expected to recognize isoform 1 and 2 (NP_065116.2; NP_001161076.1).
Predict Reactivity
Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-LIRETQPISKQH, from the C Terminus of the protein sequence according to NP_065116.2; NP_001161076.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
X-prolyl aminopeptidase 1 , APP1 , SAMP , XPNPEP , XPNPEPL , XPNPEPL1
Cellular Localization
Cytoplasm
Background
This gene encodes the cytosolic form of a metalloaminopeptidase that catalyzes the cleavage of the N-terminal amino acid adjacent to a proline residue. The gene product may play a role in degradation and maturation of tachykinins, neuropeptides, and peptide hormones. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009]
Database
Research Area
DATA IMAGES
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GTX88401 WB Image
WB analysis of pig pancreas lysate using GTX88401 XPNPEP1 antibody, C-term.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer
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GTX88401 WB Image
WB analysis of mouse (A) and rat (B) small intestine lysates using GTX88401 XPNPEP1 antibody, C-term.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer
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GTX88401 IHC-P Image
IHC-P analysis of human pancreas using GTX88401 XPNPEP1 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml
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GTX88401 WB Image
WB analysis of human pancreas lysate using GTX88401 XPNPEP1 antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer
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REFERENCE
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REVIEW
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