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XPR1 antibody [HL2632]

Recombinant antibody Comparison Orthogonal Overexpression
Anti-XPR1 antibody [HL2632] used in Western Blot (WB). GTX639088
Anti-XPR1 antibody [HL2632] used in Western Blot (WB). GTX639088
Anti-XPR1 antibody [HL2632] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639088
Anti-XPR1 antibody [HL2632] used in Western Blot (WB). GTX639088
Anti-XPR1 antibody [HL2632] used in Western Blot (WB). GTX639088
Anti-XPR1 antibody [HL2632] used in Western Blot (WB). GTX639088
Anti-XPR1 antibody [HL2632] used in Western Blot (WB). GTX639088
Anti-XPR1 antibody [HL2632] used in IHC (Paraffin sections) (IHC-P). GTX639088

Cat. No. GTX639088

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2632

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all XPR1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Observed MW

60-68 (smear), 72 kDa.

Product Note

This antibody was raised against human XPR1 Intracellular domain, and it does not cross react with human LDHB, LDHC, LDHAL6A, and LDHAL6B protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human XPR1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

xenotropic and polytropic retrovirus receptor 1 , IBGC6 , SLC53A1 , SYG1 , X3

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a receptor for the xenotropic and polytropic classes of murine leukemia viruses. The encoded protein is involved in phosphate homeostasis by mediating phosphate export from the cell. Defects in this gene have been associated with idiopathic basal ganglia calcification-6. [provided by RefSeq, Jun 2016]

Database

Research Area

DATA IMAGES

Anti-XPR1 antibody [HL2632] used in Western Blot (WB). GTX639088

GTX639088 WB Image

Comparison Orthogonal

Boiled and unboiled mouse tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with XPR1 antibody [HL2632] (GTX639088) diluted at 1:1000 and the highly cited competitor's diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-XPR1 antibody [HL2632] used in Western Blot (WB). GTX639088

GTX639088 WB Image

Comparison Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with XPR1 antibody [HL2632] (GTX639088) diluted at 1:1000 and competitor's antibody (Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-XPR1 antibody [HL2632] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639088

GTX639088 ICC/IF Image

XPR1 antibody [HL2632] detects XPR1 protein at cell membrane by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: XPR1 stained by XPR1 antibody [HL2632] (GTX639088) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-XPR1 antibody [HL2632] used in Western Blot (WB). GTX639088

GTX639088 WB Image

Orthogonal

Unboiled rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with XPR1 antibody [HL2632] (GTX639088) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-XPR1 antibody [HL2632] used in Western Blot (WB). GTX639088

GTX639088 WB Image

Orthogonal Overexpression

Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with XPR1 antibody [HL2632] (GTX639088) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-XPR1 antibody [HL2632] used in Western Blot (WB). GTX639088

GTX639088 WB Image

Orthogonal

Boiled and unboiled HCT116 whole cell (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with XPR1 antibody [HL2632] (GTX639088) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-XPR1 antibody [HL2632] used in Western Blot (WB). GTX639088

GTX639088 WB Image

Orthogonal

Boiled and unboiled mouse tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with XPR1 antibody [HL2632] (GTX639088) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-XPR1 antibody [HL2632] used in IHC (Paraffin sections) (IHC-P). GTX639088

GTX639088 IHC-P Image

XPR1 antibody [HL2632] detects XPR1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
XPR1 stained by XPR1 antibody [HL2632] (GTX639088) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations