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XRCC1 antibody [2D8]

Overexpression
Anti-XRCC1 antibody [2D8] used in IHC (Paraffin sections) (IHC-P). GTX83411
Anti-XRCC1 antibody [2D8] used in Western Blot (WB). GTX83411
Anti-XRCC1 antibody [2D8] used in IHC (Paraffin sections) (IHC-P). GTX83411
Anti-XRCC1 antibody [2D8] used in IHC (Paraffin sections) (IHC-P). GTX83411
Anti-XRCC1 antibody [2D8] used in IHC (Paraffin sections) (IHC-P). GTX83411
Anti-XRCC1 antibody [2D8] used in Flow cytometry (FACS). GTX83411
Anti-XRCC1 antibody [2D8] used in Flow cytometry (FACS). GTX83411
Anti-XRCC1 antibody [2D8] used in IHC (Paraffin sections) (IHC-P). GTX83411
Anti-XRCC1 antibody [2D8] used in Western Blot (WB). GTX83411
Anti-XRCC1 antibody [2D8] used in IHC (Paraffin sections) (IHC-P). GTX83411

Cat. No. GTX83411

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2D8

Isotype

 IgG1

Application

 WB, IHC-P, FACS

Reactivity

 Human, Monkey
Package
100 μl ($509)
See all XRCC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human XRCC1 (NP_006288) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

X-ray repair cross complementing 1 , RCC , SCAR26

Cellular Localization

Nucleus

Background

Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.

Database

Research Area

DATA IMAGES

Anti-XRCC1 antibody [2D8] used in IHC (Paraffin sections) (IHC-P). GTX83411

GTX83411 IHC-P Image

IHC-P analysis of human kidney tissue using GTX83411 XRCC1 antibody [2D8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-XRCC1 antibody [2D8] used in Western Blot (WB). GTX83411

GTX83411 WB Image

WB analysis of various cell lines using GTX83411 XRCC1 antibody [2D8].
Loading : 10 ug per lane
Dilution : 1:200

Anti-XRCC1 antibody [2D8] used in IHC (Paraffin sections) (IHC-P). GTX83411

GTX83411 IHC-P Image

IHC-P analysis of human colon adenocarcinoma tissue using GTX83411 XRCC1 antibody [2D8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-XRCC1 antibody [2D8] used in IHC (Paraffin sections) (IHC-P). GTX83411

GTX83411 IHC-P Image

IHC-P analysis of human prostate tissue using GTX83411 XRCC1 antibody [2D8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-XRCC1 antibody [2D8] used in IHC (Paraffin sections) (IHC-P). GTX83411

GTX83411 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX83411 XRCC1 antibody [2D8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-XRCC1 antibody [2D8] used in Flow cytometry (FACS). GTX83411

GTX83411 FACS Image

FACS analysis of Jurkat cells using GTX83411 XRCC1 antibody [2D8].
Red : Primary antibody
Blue : Negative control antibody

Anti-XRCC1 antibody [2D8] used in Flow cytometry (FACS). GTX83411

GTX83411 FACS Image

FACS analysis of HEK293T cells transfected with either XRCC1 plasmid(Red) or empty vector control plasmid(Blue) using GTX83411 XRCC1 antibody [2D8].

Anti-XRCC1 antibody [2D8] used in IHC (Paraffin sections) (IHC-P). GTX83411

GTX83411 IHC-P Image

IHC-P analysis of human breast adenocarcinoma tissue using GTX83411 XRCC1 antibody [2D8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-XRCC1 antibody [2D8] used in Western Blot (WB). GTX83411

GTX83411 WB Image

Overexpression

WB analysis of HEK293T cells transfected with XRCC1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83411 XRCC1 antibody [2D8].
Loading : 5 ug per lane

Anti-XRCC1 antibody [2D8] used in IHC (Paraffin sections) (IHC-P). GTX83411

GTX83411 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX83411 XRCC1 antibody [2D8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

Application Reference

Yen-Yun Wang et al. Oncol Lett 2021; 21 (6) : 450 Excision repair cross-complementing group 2 upregulation is a potential predictive biomarker for oral squamous cell carcinoma recurrence.
Application : WB, IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 509