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XRCC1 antibody [N1N3]

Anti-XRCC1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111712
Anti-XRCC1 antibody [N1N3] used in Western Blot (WB). GTX111712
Anti-XRCC1 antibody [N1N3] used in Western Blot (WB). GTX111712
Anti-XRCC1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111712

Cat. No. GTX111712

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all XRCC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

293T , rat kidney

Predict Reactivity

Mouse, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human XRCC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952603

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

X-ray repair cross complementing 1 , RCC , SCAR26

Cellular Localization

Nucleus

Background

The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-XRCC1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111712

GTX111712 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS Xenograft , using XRCC1(GTX111712) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-XRCC1 antibody [N1N3] used in Western Blot (WB). GTX111712

GTX111712 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with XRCC1 antibody [N1N3] (GTX111712) diluted at 1:500.

Anti-XRCC1 antibody [N1N3] used in Western Blot (WB). GTX111712

GTX111712 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX111712 diluted at 1:1000

Anti-XRCC1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111712

GTX111712 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using XRCC1(GTX111712) antibody at 1:200 dilution.

REFERENCE

Application Reference

Islam A et al. Nucleic Acids Res 2024; 52 (5) : 2416-2433 Site-specific acetylation of polynucleotide kinase 3'-phosphatase regulates its distinct role in DNA repair pathways.
Chen SH et al. Sci Adv 2019; 5 (4) : eaav4340 Targeting dePARylation selectively suppresses DNA repair-defective and PARP inhibitor-resistant malignancies.
Valdez BC et al. Leuk Lymphoma 2017; : 43112 the parp inhibitor olaparib enhances the cytotoxicity of combined *, * and * in lymphoma cells.
Application : WB
Reactivity : Human
Chakraborty A et al. Nat Commun 2016; 7 : 13049 Classical non-homologous end-joining pathway utilizes nascent RNA for error-free double-strand break repair of transcribed genes.
Application : WB
Reactivity : Human
Nichole M. Owen et al. 0 2015; PARP inhibitor : abrogation of chromosomal radial-induced mitotic cell death and induction of multipolar division via loss of centrosome integrity.
Application : ICC/IF
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169