GeneTex

  • Country / Location Selection

United States (US)

XRCC1 antibody [N1N3]

Anti-XRCC1 antibody [N1N3] used in Western Blot (WB). GTX111712
Anti-XRCC1 antibody [N1N3] used in Western Blot (WB). GTX111712
Anti-XRCC1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111712
Anti-XRCC1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111712

Cat No. GTX111712

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($329),
25 μl ($169)
See all XRCC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

293T , A431

Predict Reactivity

Mouse, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human XRCC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952603

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

X-ray repair cross complementing 1 , RCC , SCAR26

Cellular Localization

Nucleus

Background

The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-XRCC1 antibody [N1N3] used in Western Blot (WB). GTX111712

GTX111712 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX111712 diluted at 1:1000

Anti-XRCC1 antibody [N1N3] used in Western Blot (WB). GTX111712

GTX111712 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with XRCC1 antibody [N1N3] (GTX111712) diluted at 1:500.

Anti-XRCC1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111712

GTX111712 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using XRCC1(GTX111712) antibody at 1:200 dilution.

Anti-XRCC1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111712

GTX111712 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS Xenograft , using XRCC1(GTX111712) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Chen SH et al. Sci Adv 2019; 5 (4) : eaav4340 Targeting dePARylation selectively suppresses DNA repair-defective and PARP inhibitor-resistant malignancies.
Valdez BC et al. Leuk Lymphoma 2017; : 43112 the parp inhibitor olaparib enhances the cytotoxicity of combined *, * and * in lymphoma cells.
Application : WB
Reactivity : Human
Chakraborty A et al. Nat Commun 2016; 7 : 13049 Classical non-homologous end-joining pathway utilizes nascent RNA for error-free double-strand break repair of transcribed genes.
Application : WB
Reactivity : Human
Nichole M. Owen et al. 0 2015; PARP inhibitor : abrogation of chromosomal radial-induced mitotic cell death and induction of multipolar division via loss of centrosome integrity.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for XRCC1 antibody [N1N3] (GTX111712). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169