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XRCC3 antibody [C1C3]

Anti-XRCC3 antibody [C1C3] used in Western Blot (WB). GTX100115
Anti-XRCC3 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100115

Cat No. GTX100115

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

human XRCC3-transfected 293T
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human XRCC3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

X-ray repair cross complementing 3 , CMM6

Cellular Localization

Nucleus

Background

This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene functionally complements Chinese hamster irs1SF, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents and is chromosomally unstable. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-XRCC3 antibody [C1C3] used in Western Blot (WB). GTX100115

GTX100115 WB Image

XRCC3 antibody detects XRCC3 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of DDDDK-human XRCC3-transfected 293T cells
10 % SDS-PAGE
XRCC3 antibody (GTX100115) dilution: 1:1000

Anti-XRCC3 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100115

GTX100115 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using XRCC3(GTX100115) antibody at 1:200 dilution.

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf