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XRCC3 antibody

Anti-XRCC3 antibody used in Western Blot (WB). GTX70292

Cat. No. GTX70292

Host

 Mouse

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
50 μl ($399)
See all XRCC3 products

APPLICATION

Application Note

For WB: Use at a dilution of 1:500-1,000. Block with TBST/3% non-fat milk. Optimal working concentration for specific experiments needs to be determined by researcher.

Calculated MW

38 kDa. ( Note )

Positive Control

HCT116

Product Note

Human XRCC3

PROPERTIES

Form

Liquid

Buffer

Serum diluted with PBS pH7.4

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human XRCC3 protein

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_373300

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

X-ray repair cross complementing 3 , CMM6

Cellular Localization

Nucleus , Cytoplasm , Mitochondrion

Background

This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene functionally complements Chinese hamster irs1SF, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents and is chromosomally unstable. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-XRCC3 antibody used in Western Blot (WB). GTX70292

GTX70292 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with XRCC3 antibody (GTX70292) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399