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XRCC4 antibody [4H9]

Anti-XRCC4 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83406
Anti-XRCC4 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83406
Anti-XRCC4 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83406
Anti-XRCC4 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83406
Anti-XRCC4 antibody [4H9] used in Western Blot (WB). GTX83406
Anti-XRCC4 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83406
Anti-XRCC4 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83406
Anti-XRCC4 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83406
Anti-XRCC4 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83406
Anti-XRCC4 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83406
Anti-XRCC4 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83406
Anti-XRCC4 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83406
Anti-XRCC4 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83406
Anti-XRCC4 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83406

Cat. No. GTX83406

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4H9

Isotype

 IgG1

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($519)
See all XRCC4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
IHC-P 1:50
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human XRCC4 expression vector

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_10728925

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

X-ray repair cross complementing 4 , SSMED

Cellular Localization

Nucleus

Background

Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

Database

Research Area

DATA IMAGES

Anti-XRCC4 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83406

GTX83406 IHC-P Image

IHC-P analysis of endometrium adenocarcinoma tissue using GTX83406 XRCC4 antibody [4H9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-XRCC4 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83406

GTX83406 IHC-P Image

IHC-P analysis of colon adenocarcinoma tissue using GTX83406 XRCC4 antibody [4H9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-XRCC4 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83406

GTX83406 IHC-P Image

IHC-P analysis of thyroid carcinoma tissue using GTX83406 XRCC4 antibody [4H9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-XRCC4 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83406

GTX83406 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX83406 XRCC4 antibody [4H9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-XRCC4 antibody [4H9] used in Western Blot (WB). GTX83406

GTX83406 WB Image

WB analysis of HEK293T cells transfected with XRCC4 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83406 XRCC4 antibody [4H9].
Loading : 5 ug per lane

Anti-XRCC4 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83406

GTX83406 IHC-P Image

IHC-P analysis of endometrium tissue using GTX83406 XRCC4 antibody [4H9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-XRCC4 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83406

GTX83406 IHC-P Image

IHC-P analysis of colon tissue using GTX83406 XRCC4 antibody [4H9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-XRCC4 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83406

GTX83406 IHC-P Image

IHC-P analysis of breast adenocarcinoma tissue using GTX83406 XRCC4 antibody [4H9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-XRCC4 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83406

GTX83406 IHC-P Image

IHC-P analysis of thyroid tissue using GTX83406 XRCC4 antibody [4H9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-XRCC4 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83406

GTX83406 IHC-P Image

IHC-P analysis of lung tissue using GTX83406 XRCC4 antibody [4H9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-XRCC4 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83406

GTX83406 IHC-P Image

IHC-P analysis of bladder carcinoma tissue using GTX83406 XRCC4 antibody [4H9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-XRCC4 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83406

GTX83406 IHC-P Image

IHC-P analysis of prostate carcinoma tissue using GTX83406 XRCC4 antibody [4H9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-XRCC4 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83406

GTX83406 IHC-P Image

IHC-P analysis of kidney tissue using GTX83406 XRCC4 antibody [4H9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-XRCC4 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83406

GTX83406 IHC-P Image

IHC-P analysis of ovary tissue using GTX83406 XRCC4 antibody [4H9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

Application Reference

Prensner JR et al. Cancer Res 2014; 74 (6) : 1651-60 PCAT-1, a Long Noncoding RNA, Regulates BRCA2 and Controls Homologous Recombination in Cancer.
Application : WB
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519