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XRCC4 antibody

Anti-XRCC4 antibody used in Western Blot (WB). GTX01029
Anti-XRCC4 antibody used in IHC (Paraffin sections) (IHC-P). GTX01029

Cat No. GTX01029

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.5μg/ml
IHC-P 0.5-1μg/ml

Note :

IHC-P
Antigen retreival by heat

Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Specificity/Sensitivity

Superfamily members of XRCC4 are not reactive to this antibody.

PROPERTIES

Form

Liquid

Buffer

5mg BSA, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human XRCC4 (49-75aa ESEISQEADDMAMEKGKYVGELRKALL).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

X-ray repair cross complementing 4,SSMED,XRCC4,Xray repair cross complementing 4

Cellular Localization

Nucleus

Background

The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternate transcript variants such as NM_022406 are unlikely to be expressed in some individuals due to a polymorphism (rs1805377) in the last splice acceptor site. [provided by RefSeq, Oct 2019]

Database

Research Area

DATA IMAGES

Anti-XRCC4 antibody used in Western Blot (WB). GTX01029

GTX01029 WB Image

WB analysis of various sample lysates using GTX01029 XRCC4 antibody.
Lane 1 : SW620 cell lysate
Lane 2 : A431 cell lysate
Dilution : 0.5μg/ml

Anti-XRCC4 antibody used in IHC (Paraffin sections) (IHC-P). GTX01029

GTX01029 IHC-P Image

IHC-P analysis of human intestinal cancer tissue using GTX01029 XRCC4 antibody.
Dilution : 1μg/ml

REFERENCE

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REVIEW

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Package List Price ($)
$ 339