GeneTex

  • Country / Location Selection

United States (US)

XRCC4 antibody

Orthogonal
Anti-XRCC4 antibody used in Western Blot (WB). GTX109632
Anti-XRCC4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109632
Anti-XRCC4 antibody used in IHC (Paraffin sections) (IHC-P). GTX109632
Anti-XRCC4 antibody used in Western Blot (WB). GTX109632

Cat. No. GTX109632

Reference 8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($419),
25 μl ($169)
See all XRCC4 products
PRODUCT

Summary

XRCC4 antibody detects X-ray repair cross-complementing protein 4 (XRCC4; predicted molecular weight ~38 kDa), a crucial component of the canonical non-homologous end-joining pathway to repair DNA double-strand breaks. The protein functions by stabilizing and activating DNA Ligase IV, as well as bridging the DNA ends. Mutations or disruption of XRCC4 have been linked to a large number of cancers as well as to a form of dwarfism.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.09 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human XRCC4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952609

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

X-ray repair cross complementing 4 , SSMED

Cellular Localization

Nucleus

Background

The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. The non-homologous end-joining pathway is required both for normal development and for suppression of tumors. This gene functionally complements XR-1 Chinese hamster ovary cell mutant, which is impaired in DNA double-strand breaks produced by ionizing radiation and restriction enzymes. Alternative transcription initiation and alternative splicing generates several transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-XRCC4 antibody used in Western Blot (WB). GTX109632

GTX109632 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with XRCC4 antibody (GTX109632) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-XRCC4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109632

GTX109632 ICC/IF Image

XRCC4 antibody detects XRCC4 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: XRCC4 stained by XRCC4 antibody (GTX109632) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-XRCC4 antibody used in IHC (Paraffin sections) (IHC-P). GTX109632

GTX109632 IHC-P Image

XRCC4 antibody detects XRCC4 protein at nucleus on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
XRCC4 antibody (GTX109632) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-XRCC4 antibody used in Western Blot (WB). GTX109632

GTX109632 WB Image

Orthogonal

The data was published in the journal PLoS One in 2013. PMID: 23405159

REFERENCE

Application Reference

Kompella P et al. Nat Commun 2024; 15 (1) : 6213 Obesity increases genomic instability at DNA repeat-mediated endogenous mutation hotspots.
Islam A et al. Nucleic Acids Res 2024; 52 (5) : 2416-2433 Site-specific acetylation of polynucleotide kinase 3'-phosphatase regulates its distinct role in DNA repair pathways.
Chakraborty A et al. bioRxiv 2023; A glycolytic metabolite restores DNA repair activity of polynucleotide kinase 3'-phosphatase in polyglutamine (PolyQ) diseases.
Ali Bakr et al. Nucleic Acids Res 2021; 49 (20) : 11666-11689 ID3 promotes homologous recombination via non-transcriptional and transcriptional mechanisms and its loss confers sensitivity to PARP inhibition.
Application : WB
Reactivity : Human
Chakraborty A et al. Nat Commun 2016; 7 : 13049 Classical non-homologous end-joining pathway utilizes nascent RNA for error-free double-strand break repair of transcribed genes.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

There are currently no reviews for XRCC4 antibody (GTX109632). Be the first to share your experience with this product.
Submit a Review
Application Reference
Kompella P et al. Nat Commun 2024; 15 (1):6213 Obesity increases genomic instability at DNA repeat-mediated endogenous mutation hotspots.
Islam A et al. Nucleic Acids Res 2024; 52 (5):2416-2433 Site-specific acetylation of polynucleotide kinase 3'-phosphatase regulates its distinct role in DNA repair pathways.
Chakraborty A et al. bioRxiv 2023; A glycolytic metabolite restores DNA repair activity of polynucleotide kinase 3'-phosphatase in polyglutamine (PolyQ) diseases.
Ali Bakr et al. Nucleic Acids Res 2021; 49 (20):11666-11689 ID3 promotes homologous recombination via non-transcriptional and transcriptional mechanisms and its loss confers sensitivity to PARP inhibition.
Application : WB
Reactivity : Human
Chakraborty A et al. Nat Commun 2016; 7:13049 Classical non-homologous end-joining pathway utilizes nascent RNA for error-free double-strand break repair of transcribed genes.
Application : WB
Reactivity : Human
Chakraborty A et al. J Biol Chem. 2015; 290 (41):24636-48 Neil2-null Mice Accumulate Oxidized DNA Bases in the Transcriptionally Active Sequences of the Genome and Are Susceptible to Innate Inflammation.
Application : WB
Reactivity : Mouse
Truong LN et al. J Biol Chem 2014; 289 (42):28910-23 Homologous recombination is a primary pathway to repair DNA double-strand breaks generated during DNA rereplication.
Application : WB
Reactivity : Human
Sousa MM et al. PLoS One 2013; 8 (2):e55493 An inverse switch in DNA base excision and strand break repair contributes to * resistance in multiple myeloma cells.
Application : WB
Reactivity : Human
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169