XRCC4 antibody

Name: XRCC4 antibody
Brand: GeneTex
SKU: GTX109632
Price: 419.00 USD

Cat. No. GTX109632

GTX109632 IHC-P Image

XRCC4 antibody detects XRCC4 protein at nucleus on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
XRCC4 antibody (GTX109632) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

1 / 4

GTX109632 ICC/IF Image

XRCC4 antibody detects XRCC4 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: XRCC4 stained by XRCC4 antibody (GTX109632) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

2 / 4

GTX109632 WB Image

HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with XRCC4 antibody (GTX109632) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

3 / 4

GTX109632 WB Image

The data was published in the journal PLoS One in 2013. PMID: 23405159

4 / 4

This XRCC4 antibody (GTX109632) is a rabbit polyclonal antibody validated for detection of XRCC4 by WB, ICC/IF, and IHC-P. Reactivity has been shown in Human and Mouse. This product is cited in 11 published studies.

Host

Rabbit
Clonality

Polyclonal
Isotype

IgG
Applications

WB ICC/IF IHC-P
Reactivity

Human, Mouse

Summary

XRCC4 antibody detects X-ray repair cross-complementing protein 4 (XRCC4; predicted molecular weight ~38 kDa), a crucial component of the canonical non-homologous end-joining pathway to repair DNA double-strand breaks. The protein functions by stabilizing and activating DNA Ligase IV, as well as bridging the DNA ends. Mutations or disruption of XRCC4 have been linked to a large number of cancers as well as to a form of dwarfism.