Xanthine Oxidase antibody (HRP)

Cat. No. GTX40541

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IP, Dot, ELISA
Reactivity	Bovine
Package	1 mg ($399)

See all Xanthine Oxidase products

Datasheet

View product citations for antibody GTX40541 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:5000
IP	1:100
Dot	Assay dependent
ELISA	1:5000-1:20000

Not tested in other applications.

Calculated MW

147 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl, 1% BSA

Preservative

0.01% Gentamicin Sulfate

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

Full-length Xanthine Oxidase from bovine buttermilk

Purification

IgG fraction
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

xanthine dehydrogenase , XOR

Cellular Localization

Cytoplasm,Secreted

Background

In normal tissues this enzyme exists predominantly as Xanthine Dehydrogenase which converts hypoxanthine and xanthine to uric acid, a critical plasma antioxidant. During ischemia, Xanthine Dehydrogenase is reversibly converted to the oxygen radical-producing Xanthine Oxidase by oxidation of essential sulfhydryl groups, or irreversibly, through limited proteolytic cleavage of the amino-terminus. Aldehyde Oxidase has a higher affinity for aldehydes than purines, but produces O2- in a manner very similar to Xanthine Oxidase because both oxidases possess nearly identical internal electron transport systems.

Database

Gene ID: 280960 XDH
UniProt: P80457 XDH

Research Area

DATA IMAGES

GTX40541 Dot Image

Dot blot analysis of bovine xanthine oxidase protein using GTX40541 Xanthine Oxidase antibody (HRP).
Lane 1 : 100 ng
Lane 2 : 33.33 ng
Lane 3 : 11.11 ng
Lane 4 : 3.7 ng
Lane 5 : 1.23 ng
Dilution : 1 μg/mL

REFERENCE

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REVIEW

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Package

List Price ($)

1 mg

$ 399

Qty