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Xanthine Oxidase antibody

Anti-Xanthine Oxidase antibody used in IHC (Paraffin sections) (IHC-P). GTX00652
Anti-Xanthine Oxidase antibody used in Flow cytometry (FACS). GTX00652
Anti-Xanthine Oxidase antibody used in IHC (Paraffin sections) (IHC-P). GTX00652
Anti-Xanthine Oxidase antibody used in Western Blot (WB). GTX00652

Cat No. GTX00652

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

Human, Mouse, Rat
Package
100 μg ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.5μg/ml
ICC/IF 0.5-1μg/ml
IHC-P 0.5-1μg/ml
IHC-Fr 0.5-1μg/ml
FACS 1-3μg/1x10⁶ cells
Not tested in other applications.

Calculated MW

146 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

5mg BSA, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E. coli-derived human Xanthine Oxidase recombinant protein (Position: T2-K343).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

XAN1 , XDH , XO , XOR , xanthine dehydrogenase , Xanthine oxidase , Xanthine dehydrogenase

Cellular Localization

Cytoplasm,Peroxisome,Secreted

Background

Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism. [provided by RefSeq, Jan 2014]

Database

Research Area

DATA IMAGES

Anti-Xanthine Oxidase antibody used in IHC (Paraffin sections) (IHC-P). GTX00652

GTX00652 IHC-P Image

IHC-P analysis of rat intestine tissue using GTX00652 Xanthine Oxidase antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1 μg/ml

Anti-Xanthine Oxidase antibody used in Flow cytometry (FACS). GTX00652

GTX00652 FACS Image

FACS analysis of A431 cells using GTX00652 Xanthine Oxidase antibody.
Blue : Primary antibody
Red : Blank control
Green : Isotype control
Antibody amount : 1μg/1x10⁶ cells for 30 min at 20ºC

Anti-Xanthine Oxidase antibody used in IHC (Paraffin sections) (IHC-P). GTX00652

GTX00652 IHC-P Image

IHC-P analysis of mouse intestine tissue using GTX00652 Xanthine Oxidase antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1 μg/ml

Anti-Xanthine Oxidase antibody used in Western Blot (WB). GTX00652

GTX00652 WB Image

WB analysis of various samples using GTX00652 Xanthine Oxidase antibody.
Lane 1 : rat liver tissue lysate
Lane 2 : mouse liver tissue lysate
Dilution : 0.5μg/ml
Loading : 50μg per lane

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 329