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YAP1 antibody [1A12]

Anti-YAP1 antibody [1A12] used in Western Blot (WB). GTX60554
Anti-YAP1 antibody [1A12] used in Flow cytometry (FACS). GTX60554
Anti-YAP1 antibody [1A12] used in IHC (Paraffin sections) (IHC-P). GTX60554
Anti-YAP1 antibody [1A12] used in IHC (Paraffin sections) (IHC-P). GTX60554
Anti-YAP1 antibody [1A12] used in ELISA (ELISA). GTX60554
Anti-YAP1 antibody [1A12] used in Western Blot (WB). GTX60554

Cat. No. GTX60554

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1A12

Isotype

 IgG1

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Human, Rat, Monkey
Package
100 μg ($409)
See all YAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human YAP1 expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Yes associated protein 1 , COB1 , YAP , YAP2 , YAP65 , YKI

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-YAP1 antibody [1A12] used in Western Blot (WB). GTX60554

GTX60554 WB Image

WB analysis of HeLa (1), C6 (2) and Cos7 (3) cell lysate using GTX60554 YAP1 antibody [1A12].

Anti-YAP1 antibody [1A12] used in Flow cytometry (FACS). GTX60554

GTX60554 FACS Image

FACS analysis of HeLa cells using GTX60554 YAP1 antibody [1A12].
Green : YAP1
Red : negative control

Anti-YAP1 antibody [1A12] used in IHC (Paraffin sections) (IHC-P). GTX60554

GTX60554 IHC-P Image

IHC-P analysis of prostate cancer tissue using GTX60554 YAP1 antibody [1A12].

Anti-YAP1 antibody [1A12] used in IHC (Paraffin sections) (IHC-P). GTX60554

GTX60554 IHC-P Image

IHC-P analysis of rectum cancer tissue using GTX60554 YAP1 antibody [1A12].

Anti-YAP1 antibody [1A12] used in ELISA (ELISA). GTX60554

GTX60554 ELISA Image

ELISA analysis of antigen using GTX60554 YAP1 antibody [1A12].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-YAP1 antibody [1A12] used in Western Blot (WB). GTX60554

GTX60554 WB Image

WB analysis of human YAP1 (AA: 250-447) recombinant protein using GTX60554 YAP1 antibody [1A12].

REFERENCE

Application Reference

Chang HA et al. Cell Death Discov 2023; 9 (1) : 385 YAP nuclear translocation induced by HIF-1α prevents DNA damage under hypoxic conditions.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409