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YAP1 antibody

Anti-YAP1 antibody used in Western Blot (WB). GTX129151
Anti-YAP1 antibody used in Western Blot (WB). GTX129151
Anti-YAP1 antibody used in Immunoprecipitation (IP). GTX129151
Anti-YAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129151
Anti-YAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX129151
Anti-YAP1 antibody used in Western Blot (WB). GTX129151

Cat No. GTX129151

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , YAP1-transfected 293T

Predict Reactivity

Mouse, Rat, Zebrafish, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human YAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Yes associated protein 1 , COB1 , YAP , YAP2 , YAP65 , YKI

Background

This gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-YAP1 antibody used in Western Blot (WB). GTX129151

GTX129151 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with YAP1 antibody (GTX129151) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-YAP1 antibody used in Western Blot (WB). GTX129151

GTX129151 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with YAP1 antibody (GTX129151) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-YAP1 antibody used in Immunoprecipitation (IP). GTX129151

GTX129151 IP Image

YAP1 antibody immunoprecipitates YAP1 protein in IP experiments.
IP samples: HeLa whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of YAP1 protein by 4 μg YAP1 antibody (GTX129151)
7.5 % SDS-PAGE
The immunoprecipitated YAP1 protein was detected by YAP1 antibody (GTX129151) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-YAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129151

GTX129151 ICC/IF Image

YAP1 antibody detects YAP1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: YAP1 protein stained by YAP1 antibody (GTX129151) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-YAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX129151

GTX129151 IHC-P Image

YAP1 antibody detects YAP1 protein at nucleus on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
YAP1 antibody (GTX129151) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-YAP1 antibody used in Western Blot (WB). GTX129151

GTX129151 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with YAP1 antibody (GTX129151) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf