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YAP1 (phospho Ser127) antibody

Anti-YAP1 (phospho Ser127) antibody used in ELISA (ELISA). GTX04728
Anti-YAP1 (phospho Ser127) antibody used in IHC (Paraffin sections) (IHC-P). GTX04728
Anti-YAP1 (phospho Ser127) antibody used in IHC (Paraffin sections) (IHC-P). GTX04728
Anti-YAP1 (phospho Ser127) antibody used in Western Blot (WB). GTX04728

Cat. No. GTX04728

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all YAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
ELISA Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Predict Reactivity

Zebrafish, Rabbit, Sheep, Chicken, Pig, Monkey, Horse, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150 mM NaCl, 50% glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human YAP1(Accession P46937), corresponding to amino acid residues around phosphorylated Ser127.

Purification


From serum

Purity

Purified by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

COB1,YAP,YAP1,YAP2,YAP65,YKI,Yes associated protein 1

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013]

Database

Research Area

DATA IMAGES

Anti-YAP1 (phospho Ser127) antibody used in ELISA (ELISA). GTX04728

GTX04728 ELISA Image

ELISA analysis of phospho- and non phosho- immunogen peptide using serial diluted GTX04728 YAP1 (phospho Ser127) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1 μg/mL

Anti-YAP1 (phospho Ser127) antibody used in IHC (Paraffin sections) (IHC-P). GTX04728

GTX04728 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX04728 YAP1 (phospho Ser127) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

Anti-YAP1 (phospho Ser127) antibody used in IHC (Paraffin sections) (IHC-P). GTX04728

GTX04728 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX04728 YAP1 (phospho Ser127) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

Anti-YAP1 (phospho Ser127) antibody used in Western Blot (WB). GTX04728

GTX04728 WB Image

WB analysis of UV-treated MCF-7 whole cell lysate using GTX04728 YAP1 (phospho Ser127) antibody. The lane on the left was treated with blocking peptide.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399