APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000 |
| 1:10-1:50 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat, Bovine, Xenopus(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
KLH conjugated synthetic peptide between 276-305 amino acids from the C-terminal region of human YBX1.
Purification
Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Y-box binding protein 1 , BP-8 , CBF-A , CSDA2 , CSDB , DBPB , EFI-A , MDR-NF1 , NSEP-1 , NSEP1 , YB-1 , YB1
Cellular Localization
Cytoplasm,Nucleus,Secreted
Background
This gene encodes a highly conserved cold shock domain protein that has broad nucleic acid binding properties. The encoded protein functions as both a DNA and RNA binding protein and has been implicated in numerous cellular processes including regulation of transcription and translation, pre-mRNA splicing, DNA reparation and mRNA packaging. This protein is also a component of messenger ribonucleoprotein (mRNP) complexes and may have a role in microRNA processing. This protein can be secreted through non-classical pathways and functions as an extracellular mitogen. Aberrant expression of the gene is associated with cancer proliferation in numerous tissues. This gene may be a prognostic marker for poor outcome and drug resistance in certain cancers. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on multiple chromosomes. [provided by RefSeq, Sep 2015]
Database
Research Area
DATA IMAGES
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GTX81909 ICC/IF Image
ICC/IF analysis of U251 cells using GTX81909 YB1 antibody, C-term.
Green : YB1
Red : Actin filament
Blue : cell nucleus
Fixation : 4% paraformaldehyde (PFA) (20 min)
Permeabilization : Triton X-100 (0.1%, 10 min)
Dilution : 1:25
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GTX81909 WB Image
WB analysis of U-251 MG cell lysate using GTX81909 YB1 antibody, C-term.
Loading : 35ug
Dilution : 1:1000
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GTX81909 WB Image
WB analysis of 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the YB1 (Lane 2) using GTX81909 YB1 antibody, C-term.
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GTX81909 WB Image
WB analysis of 293 cell lysate (35ug/lane) using GTX81909 YB1 antibody, C-term.
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REFERENCE
REVIEW
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