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YBX2 antibody [N2C2], Internal

Anti-YBX2 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116127
Anti-YBX2 antibody [N2C2], Internal used in Western Blot (WB). GTX116127
Anti-YBX2 antibody [N2C2], Internal used in Western Blot (WB). GTX116127
Anti-YBX2 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX116127

Cat. No. GTX116127

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all YBX2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

IMR32 , mouse testis

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human YBX2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166982

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Y-box binding protein 2 , CONTRIN , CSDA3 , DBPC , MSY2

Cellular Localization

Cytoplasm , Nucleus

Background

YBX2 is a germ-cell specific member of the vertebrate Y box family of nucleic acid-binding proteins (Gu et al., 1998 [PubMed 9780336]; Tekur et al., 1999 [PubMed 10100484]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-YBX2 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116127

GTX116127 ICC/IF Image

YBX2 antibody [N2C2], Internal detects YBX2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: YBX2 protein stained by YBX2 antibody [N2C2], Internal (GTX116127) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-YBX2 antibody [N2C2], Internal used in Western Blot (WB). GTX116127

GTX116127 WB Image

Sample (50 ug of whole cell lysate)
A: mouse testis
10% SDS PAGE
GTX116127 diluted at 1:1000

Anti-YBX2 antibody [N2C2], Internal used in Western Blot (WB). GTX116127

GTX116127 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
10% SDS PAGE
GTX116127 diluted at 1:1000

Anti-YBX2 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX116127

GTX116127 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using YBX2(GTX116127) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169