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YBX2 antibody

Anti-YBX2 antibody used in Western Blot (WB). GTX85161
Anti-YBX2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85161
Anti-YBX2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85161

Cat. No. GTX85161

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all YBX2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

Human Testis Tissue Lysate(GTX15374) , Human Testis Tissue Slide(GTX21748)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

YBX2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy end of human YBX2.The immunogen is located within amino acids 300 - 350 of YBX2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Y-box binding protein 2 , CONTRIN , CSDA3 , DBPC , MSY2

Cellular Localization

Cytoplasm,Nucleus

Background

Germ cell specific Y-box binding protein (YBX2), a germ-cell-specific member of the Y-box family of DNA-/RNA-binding proteins, is a major constituent of messenger ribonucleoprotein particles (mRNPs) and involved in the regulation of the stability and/or translation of germ cell mRNAs. It is proposed to function as a coactivator of transcription in the nucleus and to stabilize and store maternal and paternal mRNAs in the cytoplasm. YBX2 binds to the Y-box consensus promoter element and is expressed in oocytes and testicular germ cells in the stage of spermatogonia to spermatocyte. Recent studies show that deletion of YBX2 leads to the disruption of both spermatogenesis and oogenesis.

Database

Research Area

DATA IMAGES

Anti-YBX2 antibody used in Western Blot (WB). GTX85161

GTX85161 WB Image

WB analysis of human testis tissue lysate using GTX85161 YBX2 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-YBX2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85161

GTX85161 IHC-P Image

IHC-P analysis of human testis tissue using GTX85161 YBX2 antibody.
Working concentration : 20 μg/ml

Anti-YBX2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85161

GTX85161 IHC-P Image

IHC-P analysis of human testis tissue using GTX85161 YBX2 antibody.
Working concentration : 10 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399