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YTHDC2 antibody [HL1861]

Recombinant antibody Orthogonal
Anti-YTHDC2 antibody [HL1861] used in Western Blot (WB). GTX637586
Anti-YTHDC2 antibody [HL1861] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637586
Anti-YTHDC2 antibody [HL1861] used in Western Blot (WB). GTX637586

Cat. No. GTX637586

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1861

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all YTHDC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

160 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human YTHDC2

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

YTH domain containing 2 , CAHL , hYTHDC2

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the DEAH (Asp-Glu-Ala-His) subfamily of proteins, part of the DEAD (Asp-Glu-Ala-Asp) box family of RNA helicases. The encoded protein binds to N6-methyladenosine, a common modified RNA nucleotide that is enriched in the stop codons and 3' UTRs of eukaryotic messenger RNAs. Binding of proteins to this modified nucleotide may regulate mRNA translation and stability. This gene may be associated with susceptibility to pancreatic cancer in human patients, and knockdown of this gene resulted in reduced proliferation in a human liver cancer cell line. [provided by RefSeq, Sep 2016]

Database

Research Area

DATA IMAGES

Anti-YTHDC2 antibody [HL1861] used in Western Blot (WB). GTX637586

GTX637586 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with YTHDC2 antibody [HL1861] (GTX637586) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-YTHDC2 antibody [HL1861] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637586

GTX637586 ICC/IF Image

YTHDC2 antibody [HL1861] detects YTHDC2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: YTHDC2 stained by YTHDC2 antibody [HL1861] (GTX637586) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-YTHDC2 antibody [HL1861] used in Western Blot (WB). GTX637586

GTX637586 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with YTHDC2 antibody [HL1861] (GTX637586) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations