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YY1 antibody [GT1162]

Recombinant antibody
Anti-YY1 antibody [GT1162] used in Western Blot (WB). GTX08969
Anti-YY1 antibody [GT1162] used in Western Blot (WB). GTX08969
Anti-YY1 antibody [GT1162] used in Western Blot and Immunoprecipitation (WB IP). GTX08969
Anti-YY1 antibody [GT1162] used in ChIP assay (ChIP assay). GTX08969
Anti-YY1 antibody [GT1162] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX08969

Cat. No. GTX08969

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1162

Isotype

 IgG

Application

 WB, ICC/IF, IP, ChIP assay

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all YY1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IP Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from hmuan YY1

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

YY1 transcription factor , DELTA , GADEVS , INO80S , NF-E1 , UCRBP , YIN-YANG-1

Cellular Localization

Nucleus matrix

Background

YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-YY1 antibody [GT1162] used in Western Blot (WB). GTX08969

GTX08969 WB Image

WB analysis of various samples using GTX08969 YY1 antibody [GT1162].
Dilution : 1:1000
Loading : 25 μg

Anti-YY1 antibody [GT1162] used in Western Blot (WB). GTX08969

GTX08969 WB Image

WB analysis of various samples using GTX08969 YY1 antibody [GT1162].
Loading : 25μg
Dilution : 1:1000

Anti-YY1 antibody [GT1162] used in Western Blot and Immunoprecipitation (WB IP). GTX08969

GTX08969 WB IP Image

IP analysis of HeLa cell lysate using GTX08969 YY1 antibody [GT1162].
IP antibody amount : 3μg / 300μg extracts
Dilution : 1:2000

Anti-YY1 antibody [GT1162] used in ChIP assay (ChIP assay). GTX08969

GTX08969 ChIP assay Image

ChIP analysis of 293T cell lysate using GTX08969 YY1 antibody [GT1162]. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-YY1 antibody [GT1162] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX08969

GTX08969 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX08969 YY1 antibody [GT1162].
Red : Primary antibody
Green : α-Tubulin
Blue : DAPI
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399