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Yellow Fever virus NS5 protein antibody

Anti-Yellow Fever virus NS5 protein antibody used in Western Blot (WB). GTX134141

Cat. No. GTX134141

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Yellow Fever virus
Package
100 μl ($399),
25 μl ($169)
See all Yellow Fever virus NS5 protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Positive Control

YFV NS5-transfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Yellow Fever virus

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of Yellow Fever Virus NS5 protein (Yellow fever virus (strain 17D vaccine)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887231

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Yellow Fever Virus Non-structural protein 5 , Yellow Fever Virus NS5 , YFV Non-structural protein 5 , YFV NS5

Research Area

DATA IMAGES

Anti-Yellow Fever virus NS5 protein antibody used in Western Blot (WB). GTX134141

GTX134141 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Yellow Fever Virus NS5 protein antibody (GTX134141) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

J Shu et al. Microbiol Spectr 2023; : e0360923 Zika virus infection triggers caspase cleavage of STAT1
Application : WB
Jun Shu et al. Emerg Microbes Infect 2021; 10 (1) : 1609-1625 NS5-independent Ablation of STAT2 by Zika virus to antagonize interferon signalling.
Application : WB
Gao Z et al. Antiviral Res 2020; 182 : 104907 Development of antibody-based assays for high throughput discovery and mechanistic study of antiviral agents against yellow fever virus.
Application : WB, ICC/IF
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169
Currently in production