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Yellow Fever virus NS1 Protein antibody

Anti-Yellow Fever virus NS1 Protein antibody used in Western Blot (WB). GTX134025

Cat No. GTX134025

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Yellow Fever virus
Package
100 μl ($329),
25 μl ($169)
See all Yellow Fever virus NS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Positive Control

YFV NS1-transfected 293T

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Yellow Fever virus

Immunogen

Recombinant protein encompassing a sequence within the center region of Yellow Fever Virus NS1 Protein (Yellow fever virus (strain 17D vaccine)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887176

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Yellow Fever Virus Non-structural protein 1 , Yellow Fever Virus NS1 , YFV Non-structural protein 1 , YFV NS1

Research Area

DATA IMAGES

Anti-Yellow Fever virus NS1 Protein antibody used in Western Blot (WB). GTX134025

GTX134025 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Yellow Fever Virus NS1 Protein antibody (GTX134025) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Gao Z et al. Antiviral Res 2020; 182 : 104907 Development of antibody-based assays for high throughput discovery and mechanistic study of antiviral agents against yellow fever virus.
Application : WB, ICC/IF
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169