APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Molt-4
Predict Reactivity
Mouse, Zebrafish, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human ZAK. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1952639
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
mitogen-activated protein kinase kinase kinase 20 , AZK , CNM6 , MLK7 , MLT , MLTK , MLTKalpha , MLTKbeta , MRK , SFMMP , ZAK , mlklak , pk
Cellular Localization
Cytoplasm , Nucleus
Background
This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX111477 IHC-P Image
ZAK antibody [N1N2], N-term detects ZAK protein at on Mahlarvu xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Mahlarvu xenograft. ZAK antibody [N1N2], N-term (GTX111477) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX111477 WB Image
Sample (30 ug of whole cell lysate) A: Molt-4 (GTX27912) 7.5% SDS PAGE GTX111477 diluted at 1:1000
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GTX111477 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed A431, using ZAK(GTX111477) antibody at 1:200 dilution.
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REFERENCE
There are currently no references for ZAK antibody [N1N2], N-term (GTX111477). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ZAK antibody [N1N2], N-term (GTX111477). Be the first to share your experience with this product.