APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa
Predict Reactivity
Mouse, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.92 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human ZAK. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10617610
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
mitogen-activated protein kinase kinase kinase 20 , AZK , CNM6 , MLK7 , MLT , MLTK , MLTKalpha , MLTKbeta , MRK , SFMMP , ZAK , mlklak , pk
Cellular Localization
Cytoplasm , Nucleus
Background
This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-ZAK antibody [N3C2], Internal used in Western Blot (WB). GTX103317](/templates/default/js/lightbox/images/loading.gif "Anti-ZAK antibody [N3C2], Internal used in Western Blot (WB). GTX103317")
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GTX103317 WB Image
Wild-type (WT) and ZAK knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAK antibody [N3C2], Internal (GTX103317) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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REFERENCE
There are currently no references for ZAK antibody [N3C2], Internal (GTX103317). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ZAK antibody [N3C2], Internal (GTX103317). Be the first to share your experience with this product.
![Anti-ZAK antibody [N3C2], Internal used in Western Blot (WB). GTX103317](/upload/website/prouct_img/small/GTX103317/GTX103317_40079_20181123_WB_KO_watermark_w_23060119_470.webp "Anti-ZAK antibody [N3C2], Internal used in Western Blot (WB). GTX103317")
![Anti-ZAK antibody [N3C2], Internal used in Western Blot (WB). GTX103317](/upload/website/prouct_img/normal/GTX103317/GTX103317_40079_20181123_WB_KO_watermark_w_23060119_470.webp "Anti-ZAK antibody [N3C2], Internal used in Western Blot (WB). GTX103317")