ZAP70 antibody [2F3.2]

Cat. No. GTX35177

Host	Mouse
Clonality	Monoclonal
Clone Name	2F3.2
Isotype	IgG2a
Application	ICC/IF, IHC-P, FACS
Reactivity	Human
Package	100 μg ($529)

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Datasheet
Document

View product citations for antibody GTX35177 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
ICC/IF	1-2μg/ml
IHC-P	1-2μg/ml for 30 minutes at RT
FACS	1-2μg/10⁶ cells
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

70 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP70

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ADMIO2 , IMD48 , SRK , STCD , STD , TZK , ZAP70 , zeta chain of T cell receptor associated protein kinase 70

Cellular Localization

Cytoplasm,Cell membrane

Background

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Gene ID: 7535 ZAP70
UniProt: P43403 ZAP70

Research Area

DATA IMAGES

GTX35177 FACS Image

FACS analysis of PFA-fixed Jurkat cells using GTX35177 ZAP70 antibody [2F3.2].
Blue : Primary antibody
Red : Isotype control

GTX35177 ICC/IF Image

ICC/IF analysis of PFA-fixed Jurkat cells using GTX35177 ZAP70 antibody [2F3.2].
Green : Primary antibody
Red : nucleus

GTX35177 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX35177 ZAP70 antibody [2F3.2].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 529

Qty