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ZAP70 antibody [4B2]

Orthogonal
Anti-ZAP70 antibody [4B2] used in Western Blot (WB). GTX66846
Anti-ZAP70 antibody [4B2] used in Immunoprecipitation (IP). GTX66846

Cat. No. GTX66846

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4B2

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($459)
See all ZAP70 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IP 1:50-1:200
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.2% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human ZAP70 protein fragments expressed in E.coli.

Purification

Purified by antigen-affinity chromatography

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

zeta chain of T cell receptor associated protein kinase 70 , ADMIO2 , IMD48 , SRK , STCD , STD , TZK , ZAP-70

Cellular Localization

Cytoplasm,Cell membrane

Background

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ZAP70 antibody [4B2] used in Western Blot (WB). GTX66846

GTX66846 WB Image

Orthogonal

WB analysis of CEM, Jurkat cell, and Ramos cell lysates (negative control) using GTX66846 ZAP70 antibody [4B2].
Dilution : 1:1000

Anti-ZAP70 antibody [4B2] used in Immunoprecipitation (IP). GTX66846

GTX66846 IP Image

Orthogonal

IP analysis of Jurkat cell lysates (ZAP-70 positive expression cell line) and Ramos cell lysates (ZAP-70 negative expression cell line) using GTX66846 ZAP70 antibody [4B2].

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 459