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ZAP70 antibody [ZAP-03]

Anti-ZAP70 antibody [ZAP-03] used in Western Blot (WB). GTX00530
Anti-ZAP70 antibody [ZAP-03] used in Western Blot (WB). GTX00530

Cat. No. GTX00530

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 ZAP-03

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μg ($399)
See all ZAP70 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP70 with histidine tag

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ADMIO2 , IMD48 , SRK , STCD , STD , TZK , ZAP70 , zeta chain of T cell receptor associated protein kinase 70

Cellular Localization

Cytoplasm,Cell membrane

Background

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ZAP70 antibody [ZAP-03] used in Western Blot (WB). GTX00530

GTX00530 WB Image

WB analysis of HPB-ALL cell lysate using GTX00530 ZAP70 antibody [ZAP-03].
Lane 1, 2, 3, 4 : GTX00530 (Dilution : 4, 2, 1, 0.5 μg/ml)
Lane 5 : anti-ZAP-70 comparative antibody
Lane 6 : mouse IgG1 isotype control

Anti-ZAP70 antibody [ZAP-03] used in Western Blot (WB). GTX00530

GTX00530 WB Image

WB analysis of HPB-ALL (Lane 1, 3) and Ramos cell (Lane 2, 4) lysates using GTX00530 ZAP70 antibody [ZAP-03].
Lane 1, 2 : GTX00530
Lane 3, 4 : mouse IgG1 isotype control
Diultion : 0.5μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399