APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2 μg/ml |
| 2-4 μg/ml (for coating) |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.
ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).
|
Not tested in other applications.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
200 μg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide corresponding to human ZAP-70 residues within aa200-350
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
zeta chain of T cell receptor associated protein kinase 70 , ADMIO2 , IMD48 , SRK , STCD , STD , TZK , ZAP-70
Cellular Localization
Cytoplasm , Cell membrane
Background
This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-ZAP70 antibody [ZAP70/4040R] used in IHC (Paraffin sections) (IHC-P). GTX02746](/templates/default/js/lightbox/images/loading.gif "Anti-ZAP70 antibody [ZAP70/4040R] used in IHC (Paraffin sections) (IHC-P). GTX02746")
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GTX02746 IHC-P Image
IHC-P analysis of human tonsil tissue section using GTX02746 ZAP70 antibody [ZAP70/4040R].
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GTX02746 IHC-P Image
IHC-P analysis of human tonsil tissue section using GTX02746 ZAP70 antibody [ZAP70/4040R].
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REFERENCE
There are currently no references for ZAP70 antibody [ZAP70/4040R] (GTX02746). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ZAP70 antibody [ZAP70/4040R] (GTX02746). Be the first to share your experience with this product.
![Anti-ZAP70 antibody [ZAP70/4040R] used in IHC (Paraffin sections) (IHC-P). GTX02746](/upload/website/prouct_img/small/GTX02746/GTX02746_20210319_IHC-P_1_w_23053122_142.webp "Anti-ZAP70 antibody [ZAP70/4040R] used in IHC (Paraffin sections) (IHC-P). GTX02746")
![Anti-ZAP70 antibody [ZAP70/4040R] used in IHC (Paraffin sections) (IHC-P). GTX02746](/upload/website/prouct_img/small/GTX02746/GTX02746_20210319_IHC-P_w_23053122_348.webp "Anti-ZAP70 antibody [ZAP70/4040R] used in IHC (Paraffin sections) (IHC-P). GTX02746")
![Anti-ZAP70 antibody [ZAP70/4040R] used in IHC (Paraffin sections) (IHC-P). GTX02746](/upload/website/prouct_img/normal/GTX02746/GTX02746_20210319_IHC-P_1_w_23053122_142.webp "Anti-ZAP70 antibody [ZAP70/4040R] used in IHC (Paraffin sections) (IHC-P). GTX02746")
![Anti-ZAP70 antibody [ZAP70/4040R] used in IHC (Paraffin sections) (IHC-P). GTX02746](/upload/website/prouct_img/normal/GTX02746/GTX02746_20210319_IHC-P_w_23053122_348.webp "Anti-ZAP70 antibody [ZAP70/4040R] used in IHC (Paraffin sections) (IHC-P). GTX02746")