GeneTex
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ZAP70 antibody [ZAP70/528]

Anti-ZAP70 antibody [ZAP70/528] used in IHC (Paraffin sections) (IHC-P). GTX35179

Cat No. GTX35179

Host Mouse
Clonality Monoclonal
Clone Name ZAP70/528
Isotype IgG2a
Application ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Specificity/Sensitivity

ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells.ĀControl of this protein translation is via the IgVH gene. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well.ĀAnti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
PROPERTIES

Form

Liquid

Buffer

Prepared in 1mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant full-length human ZAP70 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Zeta Chain Of T Cell Receptor Associated Protein Kinase 70,Admio2,Imd48,Srk,Stcd,Std,Tzk,Zap-70,Zap70

Background

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-ZAP70 antibody [ZAP70/528] used in IHC (Paraffin sections) (IHC-P). GTX35179

GTX35179 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with ZAP70 Monoclonal Antibody (ZAP70/528).

Package List Price ($)
$ 329