GeneTex
United States (US)

ZAP70 antibody

GTX107537_41017_20171222_WB_19011506_124.jpg
GTX107537_41017_IFA_19011506_469.jpg

Cat No. GTX107537

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

Jurkat

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZAP70. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Zeta Chain Of T Cell Receptor Associated Protein Kinase 70,Admio2,Imd48,Srk,Stcd,Std,Tzk,Zap-70,Zap70

Cellular Localization

Cytoplasm

Background

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX107537_41017_20171222_WB_19011506_124.jpg

GTX107537 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ZAP70 antibody [N1C1] (GTX107537) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107537_41017_IFA_19011506_469.jpg

GTX107537 ICC/IF Image

ZAP70 antibody detects ZAP70 protein at cytoplasm by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: ZAP70 protein stained by ZAP70 antibody (GTX107537) diluted at 1:500.
Blue: Hoechst 33342 staining.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169