APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat
Product Note
IP/MS validation was supported by references (PMID:30377390)
Predict Reactivity
Mouse, Rat, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.21 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human ZAP70. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10730232
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
zeta chain of T cell receptor associated protein kinase 70 , ADMIO2 , IMD48 , SRK , STCD , STD , TZK , ZAP-70
Cellular Localization
Cytoplasm
Background
This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX107950 IHC-P Image
Immunohistochemical analysis of paraffin-embedded U373 xenograft, using ZAP70(GTX107950) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX107950 ICC/IF Image
ZAP70 antibody detects ZAP70 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZAP70 protein stained by ZAP70 antibody (GTX107950) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX107950 IP Image
Immunoprecipitation of ZAP70 protein from Jurkat whole cell extracts using 5 μg of ZAP70 antibody (GTX107950).
Western blot analysis was performed using ZAP70 antibody (GTX107950) diluted at 1:1000.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX107950 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ZAP70 antibody (GTX107950) diluted at 1:3000 and competitor's antibody (SC-574) diluted by 1:200.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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REFERENCE
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