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ZAP70 antibody

Anti-ZAP70 antibody used in Immunoprecipitation (IP). GTX107950
Anti-ZAP70 antibody used in Western Blot (WB). GTX107950
Anti-ZAP70 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107950
Anti-ZAP70 antibody used in IHC (Paraffin sections) (IHC-P). GTX107950

Cat No. GTX107950

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

Jurkat

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377390)

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZAP70. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

zeta chain of T cell receptor associated protein kinase 70,ADMIO2,IMD48,SRK,STCD,STD,TZK,ZAP-70

Cellular Localization

Cytoplasm

Background

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-ZAP70 antibody used in Immunoprecipitation (IP). GTX107950

GTX107950 IP Image

Immunoprecipitation of ZAP70 protein from Jurkat whole cell extracts using 5 μg of ZAP70 antibody (GTX107950).
Western blot analysis was performed using ZAP70 antibody (GTX107950).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-ZAP70 antibody used in Western Blot (WB). GTX107950

GTX107950 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ZAP70 antibody (GTX107950) diluted at 1:3000 and competitor's antibody (SC-574) diluted by 1:200.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-ZAP70 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107950

GTX107950 ICC/IF Image

ZAP70 antibody detects ZAP70 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZAP70 protein stained by ZAP70 antibody (GTX107950) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-ZAP70 antibody used in IHC (Paraffin sections) (IHC-P). GTX107950

GTX107950 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using ZAP70(GTX107950) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169