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ZAP70 (phospho Tyr292) antibody

Anti-ZAP70 (phospho Tyr292) antibody used in IHC (Paraffin sections) (IHC-P). GTX12868
Anti-ZAP70 (phospho Tyr292) antibody used in Western Blot (WB). GTX12868

Cat No. GTX12868

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
50 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P 1:20
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1mg/ml BSA, 50% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human ZAP-70 that contains tyrosine 292. The sequence is conserved in mouse.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

zeta chain of T cell receptor associated protein kinase 70 , ADMIO2 , IMD48 , SRK , STCD , STD , TZK , ZAP-70

Cellular Localization

Cytoplasm,Cell membrane

Background

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ZAP70 (phospho Tyr292) antibody used in IHC (Paraffin sections) (IHC-P). GTX12868

GTX12868 IHC-P Image

IHC-P analysis of human lymph node tissue using GTX12868 ZAP70 (phospho Tyr292) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-ZAP70 (phospho Tyr292) antibody used in Western Blot (WB). GTX12868

GTX12868 WB Image

WB (peptide competition) analysis of Jurkat cells treated with hydrogen peroxide (Lane 2-6) using GTX12868 ZAP70 (phospho Tyr292) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 2), a generic phosphotyrosine-containing peptide (Lane 4) or, the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 6) eliminates the signal further verifying that the antibody is phospho-specific.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319