IHC-P analysis of human lymph node tissue using GTX12868 ZAP70 (phospho Tyr292) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

WB (peptide competition) analysis of Jurkat cells treated with hydrogen peroxide (Lane 2-6) using GTX12868 ZAP70 (phospho Tyr292) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 2), a generic phosphotyrosine-containing peptide (Lane 4) or, the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 6) eliminates the signal further verifying that the antibody is phospho-specific.