GeneTex

  • Country / Location Selection

United States (US)

ZAP70 (phospho Tyr315/Tyr319) antibody

Orthogonal
Anti-ZAP70 (phospho Tyr315/Tyr319) antibody used in Western Blot (WB). GTX12869

Cat. No. GTX12869

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
50 μl ($399)
See all ZAP70 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human ZAP-70 that contains tyrosines 315 and 319. The sequence is conserved in mouse.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zeta chain of T cell receptor associated protein kinase 70 , ADMIO2 , IMD48 , SRK , STCD , STD , TZK , ZAP-70

Cellular Localization

Cytoplasm,Cell membrane

Background

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ZAP70 (phospho Tyr315/Tyr319) antibody used in Western Blot (WB). GTX12869

GTX12869 WB Image

Orthogonal

WB (peptide competition) analysis of Jurkat cells treated with H2O2 (Lane 2-6) using GTX12869 ZAP70 (phospho Tyr315/Tyr319) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 3), a generic phosphotyrosine-containing peptide (Lane 4) the phosphopeptide immunogen (Lane 5) or, the phosphopeptide corresponding to ZAP-70 (pY315) (Lane 6) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 7) eliminates the signal further verifying that the antibody is phospho-specific.

REFERENCE

There are currently no references for ZAP70 (phospho Tyr315/Tyr319) antibody (GTX12869). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for ZAP70 (phospho Tyr315/Tyr319) antibody (GTX12869). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399