APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Bovine, Chicken, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr319 of human ZAP70. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888461
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
zeta chain of T cell receptor associated protein kinase 70 , ADMIO2 , IMD48 , SRK , STCD , STD , TZK , ZAP-70
Cellular Localization
Cytoplasm,Cell membrane
Background
This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634484](/templates/default/js/lightbox/images/loading.gif "Anti-ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634484")
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GTX634484 ICC/IF Image
ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] detects ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) protein at cytoplasm by immunofluorescent analysis.Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) stained by ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] (GTX634484) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX634484 WB Image
Untreated (–) and treated (+) Jurkat whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] (GTX634484) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX634484 WB Image
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] (GTX634484) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX634484 WB Image
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] (GTX634484) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] (GTX634484). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] (GTX634484). Be the first to share your experience with this product.
![Anti-ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634484](/upload/website/prouct_img/small/GTX634484/GTX634484_43171_20200916_ICC_IF_w_23061202_388.webp "Anti-ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634484")
![Anti-ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] used in Western Blot (WB). GTX634484](/upload/website/prouct_img/small/GTX634484/GTX634484_43171_20191129_WB_treatment_CIP_w_23061202_641.webp "Anti-ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] used in Western Blot (WB). GTX634484")
![Anti-ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] used in Western Blot (WB). GTX634484](/upload/website/prouct_img/small/GTX634484/GTX634484_43171_20210416_WB_R_thymus_w_23061202_984.webp "Anti-ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] used in Western Blot (WB). GTX634484")
![Anti-ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] used in Western Blot (WB). GTX634484](/upload/website/prouct_img/small/GTX634484/GTX634484_43171_20210427_WB_M_thymus_w_23061202_112.webp "Anti-ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] used in Western Blot (WB). GTX634484")
![Anti-ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634484](/upload/website/prouct_img/normal/GTX634484/GTX634484_43171_20200916_ICC_IF_w_23061202_388.webp "Anti-ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634484")
![Anti-ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] used in Western Blot (WB). GTX634484](/upload/website/prouct_img/normal/GTX634484/GTX634484_43171_20191129_WB_treatment_CIP_w_23061202_641.webp "Anti-ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] used in Western Blot (WB). GTX634484")
![Anti-ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] used in Western Blot (WB). GTX634484](/upload/website/prouct_img/normal/GTX634484/GTX634484_43171_20210416_WB_R_thymus_w_23061202_984.webp "Anti-ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] used in Western Blot (WB). GTX634484")
![Anti-ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] used in Western Blot (WB). GTX634484](/upload/website/prouct_img/normal/GTX634484/GTX634484_43171_20210427_WB_M_thymus_w_23061202_112.webp "Anti-ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] used in Western Blot (WB). GTX634484")