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ZAP70 (phospho Tyr319) antibody

Anti-ZAP70 (phospho Tyr319) antibody used in Western Blot (WB). GTX38562
Anti-ZAP70 (phospho Tyr319) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX38562

Cat. No. GTX38562

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
50 μl ($399)
See all ZAP70 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Product Note

ZAP70 (phospho-Tyr319) antibody detects endogenous levels of ZAP70 only when phosphorylated at tyrosine 319.

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human ZAP70 around the phosphorylation site of tyrosine 319 (S -P-Y(p)-S-D).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zeta chain of T cell receptor associated protein kinase 70 , ADMIO2 , IMD48 , SRK , STCD , STD , TZK , ZAP-70

Cellular Localization

Cytoplasm,Cell membrane

Background

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ZAP70 (phospho Tyr319) antibody used in Western Blot (WB). GTX38562

GTX38562 WB Image

WB analysis of Jurkat cell lysate using ZAP70 antibody (Lane 1 and 2) and GTX38562 ZAP70 (phospho Tyr319) antibody(Lane 3 and 4).

Anti-ZAP70 (phospho Tyr319) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX38562

GTX38562 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX38562 ZAP70 (phospho Tyr319) antibody.
Red : Primary antibody

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399