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ZAP70 (phospho Tyr493) antibody

Anti-ZAP70 (phospho Tyr493) antibody used in IHC (Paraffin sections) (IHC-P). GTX50215
Anti-ZAP70 (phospho Tyr493) antibody used in Western Blot (WB). GTX50215

Cat No. GTX50215

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 493 (S-Y-Y(p)-T-A) derived from human ZAP70.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

zeta chain of T cell receptor associated protein kinase 70 , ADMIO2 , IMD48 , SRK , STCD , STD , TZK , ZAP-70

Cellular Localization

Cytoplasm,Cell membrane

Background

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ZAP70 (phospho Tyr493) antibody used in IHC (Paraffin sections) (IHC-P). GTX50215

GTX50215 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50215 ZAP70 (phospho Tyr493) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-ZAP70 (phospho Tyr493) antibody used in Western Blot (WB). GTX50215

GTX50215 WB Image

WB analysis of extracts from JK cells untreated or treated with anisomycin using GTX50215 ZAP70 (phospho Tyr493) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319