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ZBTB20 antibody

Orthogonal
Anti-ZBTB20 antibody used in IHC (Paraffin sections) (IHC-P). GTX121616
Anti-ZBTB20 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121616
Anti-ZBTB20 antibody used in Western Blot (WB). GTX121616
Anti-ZBTB20 antibody used in IHC (Paraffin sections) (IHC-P). GTX121616
Anti-ZBTB20 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121616
Anti-ZBTB20 antibody used in Western Blot (WB). GTX121616

Cat. No. GTX121616

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ZBTB20 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

NIH-3T3 , JC , BCL-1

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZBTB20. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177870

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zinc finger and BTB domain containing 20 , DPZF , HOF , ODA-8S , PRIMS , ZNF288

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-ZBTB20 antibody used in IHC (Paraffin sections) (IHC-P). GTX121616

GTX121616 IHC-P Image

ZBTB20 antibody detects ZBTB20 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
ZBTB20 stained by ZBTB20 antibody (GTX121616) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ZBTB20 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121616

GTX121616 ICC/IF Image

ZBTB20 antibody detects ZBTB20 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZBTB20 stained by ZBTB20 antibody (GTX121616) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ZBTB20 antibody used in Western Blot (WB). GTX121616

GTX121616 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX121616 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZBTB20 antibody used in IHC (Paraffin sections) (IHC-P). GTX121616

GTX121616 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using ZBTB20(GTX121616) antibody at 1:750 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ZBTB20 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121616

GTX121616 ICC/IF Image

ZBTB20 antibody detects ZBTB20 protein at by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZBTB20 stained by ZBTB20 antibody (GTX121616) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ZBTB20 antibody used in Western Blot (WB). GTX121616

GTX121616 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZBTB20 antibody (GTX121616) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

F Ciarpella et al. STAR Protoc 2023; 4 (3) : 102413 Generation of mouse hippocampal brain organoids from primary embryonic neural stem cells
Reactivity : Mouse
Ciarpella F et al. iScience 2021; 24 (12) : 103438 Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169