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ZC3H12A antibody [N3C3]

KO/KD Orthogonal
Anti-ZC3H12A antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110807
Anti-ZC3H12A antibody [N3C3] used in Western Blot (WB). GTX110807
Anti-ZC3H12A antibody [N3C3] used in Western Blot (WB). GTX110807

Cat. No. GTX110807

Reference 34
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS, IP, IHC

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ZC3H12A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
FACS Assay dependent
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Product Note

Knockdown/Knockout validation was supported by references (PMID:27322373)

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZC3H12A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952643

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zinc finger CCCH-type containing 12A , MCPIP , MCPIP-1 , MCPIP1 , Reg1 , dJ423B22.1

Cellular Localization

Cytoplasm , Nucleus

Background

ZC3H12A is an MCP1 (CCL2; MIM 158105)-induced protein that acts as a transcriptional activator and causes cell death of cardiomyocytes, possibly via induction of genes associated with apoptosis.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-ZC3H12A antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110807

GTX110807 ICC/IF Image

ZC3H12A antibody detects ZC3H12A protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZC3H12A stained by ZC3H12A antibody (GTX110807) diluted at 1:500.

Anti-ZC3H12A antibody [N3C3] used in Western Blot (WB). GTX110807

GTX110807 WB Image

Orthogonal

Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3H12A antibody [N3C3] (GTX110807) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZC3H12A antibody [N3C3] used in Western Blot (WB). GTX110807

GTX110807 WB Image

Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3H12A antibody [N3C3] (GTX110807) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

B Lin et al. Immunohorizons 2024; 8 (1) : 89-96 Lung Epithelial Regnase-1 Dampens Local Immune Response but Does Not Worsen Susceptibility to Klebsiella pneumoniae
Application : WB
Gorka J et al. Eur J Pharmacol 2022; : 174804 MCPIP1 regulates focal adhesion kinase and rho GTPase-dependent migration in clear cell renal cell carcinoma.
Application : WB
Reactivity : Human
Piotr K Krajewski et al. Int J Mol Sci 2021; 22 (14) MCPIP1/Regnase-1 Expression in Keratinocytes of Patients with Hidradenitis Suppurativa: Preliminary Results.
Application : WB, ICC/IF
Reactivity : Human
Gasiorek A et al. mBio 2021; 12 (3) : e0050221 Subversion of Lipopolysaccharide Signaling in Gingival Keratinocytes via MCPIP-1 Degradation as a Novel Pathogenic Strategy of Inflammophilic Pathobionts.
Application : ICC/IF
Reactivity : Human
Tomita T et al. Nat Commun 2021; 12 (1) : 3655 Extracellular mRNA transported to the nucleus exerts translation-independent function.
Application : ICC/IF
Reactivity : Human
Show More +
Submit a Reference

REVIEW

ZC3H12A antibody [N3C3] Cat. No. GTX110807

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-ZC3H12A antibody [N3C3] used in Western Blot (WB). GTX110807
Date : Anonymous submitted on 14-Apr-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : HT-29 cells with/without siReg1 treatment
Amount used : 75μg
Blocking : 5% BSA in TBS/0.05%Tween, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, >12Hr
Submit a Review
Application Reference
B Lin et al. Immunohorizons 2024; 8 (1):89-96 Lung Epithelial Regnase-1 Dampens Local Immune Response but Does Not Worsen Susceptibility to Klebsiella pneumoniae
Application : WB
Gorka J et al. Eur J Pharmacol 2022;:174804 MCPIP1 regulates focal adhesion kinase and rho GTPase-dependent migration in clear cell renal cell carcinoma.
Application : WB
Reactivity : Human
Piotr K Krajewski et al. Int J Mol Sci 2021; 22 (14) MCPIP1/Regnase-1 Expression in Keratinocytes of Patients with Hidradenitis Suppurativa: Preliminary Results.
Application : WB, ICC/IF
Reactivity : Human
Gasiorek A et al. mBio 2021; 12 (3):e0050221 Subversion of Lipopolysaccharide Signaling in Gingival Keratinocytes via MCPIP-1 Degradation as a Novel Pathogenic Strategy of Inflammophilic Pathobionts.
Application : ICC/IF
Reactivity : Human
Tomita T et al. Nat Commun 2021; 12 (1):3655 Extracellular mRNA transported to the nucleus exerts translation-independent function.
Application : ICC/IF
Reactivity : Human
Kook I et al. Nucleic Acids Res 2021; 49 (8):4456-4471 Monocyte chemoattractant protein-induced protein 1 directly degrades viral miRNAs with a specific motif and inhibits KSHV infection.
Application : WB
Reactivity : Human
Chen F et al. Cell Death Dis 2021; 12 (4):370 MCPIP1-mediated NFIC alternative splicing inhibits proliferation of triple-negative breast cancer via cyclin D1-Rb-E2F1 axis.
Application : WB
Reactivity : Human
Li Y et al. Sci Rep 2020; 10 (1):20763 MCPIP1 reduces HBV-RNA by targeting its epsilon structure.
Application : WB
Reactivity : Human
Gorka J et al. Eur J Pharmacol 2020; 888:173591 The anti-inflammatory protein MCPIP1 inhibits the development of ccRCC by maintaining high levels of tumour suppressors.
Application : WB
Reactivity : Human
Li M et al. Antiviral Res 2020; 174:104705 MCPIP1 inhibits Hepatitis B virus replication by destabilizing viral RNA and negatively regulates the virus-induced innate inflammatory responses.
Application : WB
Reactivity : Human
Miyazawa M et al. RNA 2019; 25 (11):1416-1420 Iron-induced transferrin receptor-1 mRNA destabilization: A response to "Neither miR-7-5p nor miR-141-3p is a major mediator of iron-responsive transferrin receptor-1 mRNA degradation".
Losko M et al. Cell Mol Life Sci 2019; (Epub) Integrative genomics reveal a role for MCPIP1 in adipogenesis and adipocyte metabolism.
Application : WB
Reactivity : Human
Ohgakiuchi Y et al. Biochem Biophys Res Commun 2019; (Epub) Dimethyl fumarate dampens IL-17-ACT1-TBK1 axis-mediated phosphorylation of Regnase-1 and suppresses IL-17-induced I庥B-庤 expression.
Muthumalage T et al. Toxicol Appl Pharmacol 2019;:114713 Cannabidiol differentially regulates basal and LPS-induced inflammatory responses in macrophages, lung epithelial cells, and fibroblasts.
Application : WB
Li Y et al. Cell Signal 2018; 50:43108 Pharmacological inhibition of MALT1 protease activity suppresses endothelial activation via enhancing MCPIP1 expression.
Sun Y et al. Sci Rep 2018; 8 (1):2518 Spheroid-cultured human umbilical cord-derived mesenchymal stem cells attenuate hepatic ischemia-reperfusion injury in rats.
Application : WB
Reactivity : Human
Li Y et al. Signal Transduct Target Ther 2017; 2:17066 Central role of myeloid MCPIP1 in protecting against LPS-induced inflammation and lung injury.
Application : WB
Li M et al. Med Microbiol Immunol 2017; (Epub) MCPIP1 inhibits coxsackievirus B3 replication by targeting viral RNA and negatively regulates virus-induced inflammation.
Application : WB
Losko M et al. Biochim Biophys Acta 2017; (Epub) Ectopic overexpression of MCPIP1 impairs adipogenesis by modulating microRNAs.
Application : WB
Ligeza J et al. Angiogenesis 2017; MCPIP1 contributes to clear cell renal cell carcinomas development.
Application : WB
Reactivity : Human
Li H et al. AIDS Res Hum Retroviruses 2016; 32 (2):174-7 Short Communication: Preferential Killing of HIV Latently Infected CD4(+) T Cells by MALT1 Inhibitor.
Makki MS et al. Connect Tissue Res 2016;:43112 Histone deacetylase inhibitor * (SAHA, MK0683) perturb miR-9-MCPIP1 axis to block IL-1β-induced IL-6 expression in human OA chondrocytes.
Application : WB
Reactivity : Human
Dobosz E et al. J Innate Immun 2016; (Epub) MCPIP-1, Alias Regnase-1, Controls Epithelial Inflammation by Posttranscriptional Regulation of IL-8 Production.
Application : WB
Reactivity : Human
Ruiz-Romeu E et al. J Invest Dermatol 2016; 136 (8):1599-607 MCPIP1 RNase Is Aberrantly Distributed in Psoriatic Epidermis and Rapidly Induced by IL-17A.
Application : WB, FACS
Reactivity : Human
Dong C et al. J Med Virol 2016; (Epub) Modulation of influenza A virus replication by microRNA-9 through targeting MCPIP1.
Application : WB
Reactivity : Human
Tan X et al. J Cell Physiol 2016; (Epub) MG132 Induces Expression of Monocyte Chemotactic Protein- Induced Protein 1 in Vascular Smooth Muscle Cells.
Application : WB
Reactivity : Human
Huang S et al. J Biol Chem 2015; 290 (34):20782-92 Monocyte Chemotactic Protein-induced Protein 1 and 4 Form a Complex but Act Independently in Regulation of Interleukin-6 mRNA Degradation.
Application : WB, ICC/IF, IP
Reactivity : Human
Makki MS et al. Arthritis Rheumatol. 2015; 67 (8):2117-28 MicroRNA-9 Promotion of Interleukin-6 Expression by Inhibiting Monocyte Chemoattractant Protein-Induced Protein 1 Expression in Interleukin-1β-Stimulated Human Chondrocytes.
Application : WB, ICC/IF
Reactivity : Human
Lin RJ et al. J Immunol 2014; 193 (8):4159-68 MCPIP1 suppresses hepatitis C virus replication and negatively regulates virus-induced proinflammatory cytokine responses.
Application : WB
Reactivity : Human
Skalniak L et al. Mol Cell Biochem 2014; MCPIP1 contributes to the toxicity of proteasome inhibitor MG-132 in HeLa cells by the inhibition of NF-κB.
Application : WB
Reactivity : Human
Liu S et al. Proc Natl Acad Sci U S A 2013; 110 (47):19083-8 MCPIP1 restricts HIV infection and is rapidly degraded in activated CD4+ T cells.
Application : WB
Reactivity : Human
Skalniak L et al. FEBS J 2013; 280 (11):2665-74 Proteasome inhibitor MG-132 induces MCPIP1 expression.
Lin RJ et al. Nucleic Acids Res 2013; 41 (5):3314-26 MCPIP1 ribonuclease exhibits broad-spectrum antiviral effects through viral RNA binding and degradation.
Application : WB
Reactivity : Human
Qi Y et al. FEBS Lett 2010; 584 (14):3065-72 MCP-induced protein 1 suppresses TNFalpha-induced VCAM-1 expression in human endothelial cells.
Application : IHC
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169