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ZC3H8 antibody [N1C3]

Anti-ZC3H8 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107008
Anti-ZC3H8 antibody [N1C3] used in Western Blot (WB). GTX107008
Anti-ZC3H8 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107008

Cat No. GTX107008

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZC3H8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952645

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

zinc finger CCCH-type containing 8 , Fliz1 , ZC3HDC8

Cellular Localization

Nucleus

Background

Acts as a transcriptional repressor of the GATA3 promoter. Sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene (By similarity). Induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis.

Database

Research Area

DATA IMAGES

Anti-ZC3H8 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107008

GTX107008 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using ZC3H8(GTX107008) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ZC3H8 antibody [N1C3] used in Western Blot (WB). GTX107008

GTX107008 WB Image

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
B:Hep G2 (GTX27900)
C:Raji (GTX27908)
12% SDS PAGE
GTX107008 diluted at 1:1000

Anti-ZC3H8 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107008

GTX107008 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ZC3H8(GTX107008) antibody at 1:200 dilution.

REFERENCE

There are currently no references for ZC3H8 antibody [N1C3] (GTX107008). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for ZC3H8 antibody [N1C3] (GTX107008). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169