GeneTex
United States (US)

ZC3H8 antibody [N1C3]

GTX107008_IHC_19011506_581.jpg
GTX107008_WB_19011506_830.jpg
GTX107008_IFA_19011506_636.jpg

Cat No. GTX107008

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZC3H8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Zinc Finger Ccch-Type Containing 8,Fliz1,Zc3Hdc8,Zc3H8

Cellular Localization

Nucleus

Background

Acts as a transcriptional repressor of the GATA3 promoter. Sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene (By similarity). Induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis.

Database

Research Area

DATA IMAGES
GTX107008_IHC_19011506_581.jpg

GTX107008 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using ZC3H8(GTX107008) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107008_WB_19011506_830.jpg

GTX107008 WB Image

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
B:Hep G2 (GTX27900)
C:Raji (GTX27908)
12% SDS PAGE
GTX107008 diluted at 1:1000

GTX107008_IFA_19011506_636.jpg

GTX107008 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ZC3H8(GTX107008) antibody at 1:200 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169