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ZC3HAV1 antibody [N3C2], Internal

Anti-ZC3HAV1 antibody [N3C2], Internal used in Western Blot (WB). GTX120134
Anti-ZC3HAV1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX120134
Anti-ZC3HAV1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX120134
Anti-ZC3HAV1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120134

Cat No. GTX120134

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

101 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.04 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZC3HAV1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721153

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

zinc finger CCCH-type containing, antiviral 1 , ARTD13 , FLB6421 , PARP13 , ZAP , ZC3H2 , ZC3HDC2

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a CCCH-type zinc finger protein that is thought to prevent infection by retroviruses. Studies of the rat homolog indicate that the protein may primarily function to inhibit viral gene expression and induce an innate immunity to viral infection. Alternative splicing occurs at this locus and two variants, each encoding distinct isoforms, are described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ZC3HAV1 antibody [N3C2], Internal used in Western Blot (WB). GTX120134

GTX120134 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3HAV1 antibody [N3C2], Internal (GTX120134) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZC3HAV1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX120134

GTX120134 IHC-P Image

ZC3HAV1 antibody [N3C2], Internal detects ZC3HAV1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
ZC3HAV1 stained by ZC3HAV1 antibody [N3C2], Internal (GTX120134) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ZC3HAV1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX120134

GTX120134 IHC-P Image

ZC3HAV1 antibody [N3C2], Internal detects ZC3HAV1 protein at cytoplasm in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
ZC3HAV1 antibody [N3C2], Internal (GTX120134) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ZC3HAV1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120134

GTX120134 ICC/IF Image

ZC3HAV1 antibody [N3C2], Internal detects ZC3HAV1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZC3HAV1 stained by ZC3HAV1 antibody [N3C2], Internal (GTX120134) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

REFERENCE

REVIEW

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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169