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ZCCHC4 antibody [N1N3]

Anti-ZCCHC4 antibody [N1N3] used in Western Blot (WB). GTX119809
Anti-ZCCHC4 antibody [N1N3] used in Western Blot (WB). GTX119809
Anti-ZCCHC4 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119809
Anti-ZCCHC4 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX119809

Cat. No. GTX119809

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ZCCHC4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

Jurkat

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZCCHC4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10720167

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zinc finger CCHC-type containing 4 , HSPC052 , ZGRF4

Background

May be a methyltransferase.

Database

Research Area

DATA IMAGES

Anti-ZCCHC4 antibody [N1N3] used in Western Blot (WB). GTX119809

GTX119809 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZCCHC4 antibody [N1N3] (GTX119809) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ZCCHC4 antibody [N1N3] used in Western Blot (WB). GTX119809

GTX119809 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZCCHC4 antibody [N1N3] (GTX119809) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ZCCHC4 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119809

GTX119809 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using ZCCHC4(GTX119809) antibody at 1:500 dilution.

Anti-ZCCHC4 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX119809

GTX119809 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using ZCCHC4(GTX119809) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169