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ZEB1 antibody [HL2245]

Recombinant antibody KO/KD Comparison Orthogonal
Anti-ZEB1 antibody [HL2245] used in Western Blot (WB). GTX638294
Anti-ZEB1 antibody [HL2245] used in Western Blot (WB). GTX638294
Anti-ZEB1 antibody [HL2245] used in Western Blot (WB). GTX638294
Anti-ZEB1 antibody [HL2245] used in IHC (Paraffin sections) (IHC-P). GTX638294
Anti-ZEB1 antibody [HL2245] used in IHC (Paraffin sections) (IHC-P). GTX638294
Anti-ZEB1 antibody [HL2245] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638294
Anti-ZEB1 antibody [HL2245] used in Western Blot (WB). GTX638294
Anti-ZEB1 antibody [HL2245] used in Western Blot (WB). GTX638294

Cat. No. GTX638294

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2245

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ZEB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human ZEB1.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zinc finger E-box binding homeobox 1 , AREB6 , BZP , DELTAEF1 , FECD6 , NIL2A , PPCD3 , TCF8 , ZFHEP , ZFHX1A

Cellular Localization

Nucleus

Background

This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-ZEB1 antibody [HL2245] used in Western Blot (WB). GTX638294

GTX638294 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (GTX638294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZEB1 antibody [HL2245] used in Western Blot (WB). GTX638294

GTX638294 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with ZEB1 antibody [HL2245] (GTX638294) diluted at 1:1000 and competitor's antibody (#Highly competitor antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.
The observed M.W. is based on the publication: PMID: 32488136

Anti-ZEB1 antibody [HL2245] used in Western Blot (WB). GTX638294

GTX638294 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (GTX638294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZEB1 antibody [HL2245] used in IHC (Paraffin sections) (IHC-P). GTX638294

GTX638294 IHC-P Image

ZEB1 antibody [HL2245] detects ZEB1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
ZEB1 stained by ZEB1 antibody [HL2245] (GTX638294) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ZEB1 antibody [HL2245] used in IHC (Paraffin sections) (IHC-P). GTX638294

GTX638294 IHC-P Image

ZEB1 antibody [HL2245] detects ZEB1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
ZEB1 stained by ZEB1 antibody [HL2245] (GTX638294) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ZEB1 antibody [HL2245] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638294

GTX638294 ICC/IF Image

ZEB1 antibody [HL2245] detects ZEB1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZEB1 stained by ZEB1 antibody [HL2245] (GTX638294) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-ZEB1 antibody [HL2245] used in Western Blot (WB). GTX638294

GTX638294 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (GTX638294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-ZEB1 antibody [HL2245] used in Western Blot (WB). GTX638294

GTX638294 WB Image

Orthogonal

(30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (GTX638294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
The observed M.W. is based on the publication: PMID: 32488136

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations