GeneTex
United States (US)

ZEB1 antibody, Internal

GTX88467_2290_WB_18121411_676.jpg

Cat No. GTX88467

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.05-0.2μg/ml
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88467-PEP).

Specificity/Sensitivity

This antibody is expected to recognize both reported isoforms (NP_001121600.1; NP_110378.3).

Predict Reactivity

Human, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-RPQIRQKIENKPLQE, from the internal region of the protein sequence according to NP_001121600.1; NP_110378.3.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Zinc Finger E-Box Binding Homeobox 1,Areb6,Bzp,Deltaef1,Fecd6,Nil2A,Ppcd3,Tcf8,Zfhep,Zfhx1A,Zeb1

Cellular Localization

Nucleus

Background

This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES
GTX88467_2290_WB_18121411_676.jpg

GTX88467 WB Image

WB analysis of mouse heart lysate using GTX88467 ZEB1 antibody, Internal.
Dilution : 0.05µg/ml
Loading : 35µg protein in RIPA buffer