GeneTex
  • Country / Location Selection

United States (US)

ZEB1 antibody [N2C1], Internal

Anti-ZEB1 antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX105278
Anti-ZEB1 antibody [N2C1], Internal used in Western Blot (WB). GTX105278
Anti-ZEB1 antibody [N2C1], Internal used in Western Blot (WB). GTX105278
Anti-ZEB1 antibody [N2C1], Internal used in ChIP assay (ChIP assay). GTX105278
Anti-ZEB1 antibody [N2C1], Internal used in Western Blot (WB). GTX105278
Anti-ZEB1 antibody [N2C1], Internal used in Western Blot (WB). GTX105278
Anti-ZEB1 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105278

Cat No. GTX105278

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP, ChIP assay

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

Positive Control

HeLa , 293T , HepG2 , MDA-MB-231 , Neuro2A , NIH-3T3 , C2C12 , ZEB1 shRNA-transfected HeLa

Predict Reactivity

Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZEB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11162905

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

zinc finger E-box binding homeobox 1 , AREB6 , BZP , DELTAEF1 , FECD6 , NIL2A , PPCD3 , TCF8 , ZFHEP , ZFHX1A

Cellular Localization

Nucleus

Background

ZEB1 encodes a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene (MIM 147680) expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (Williams et al., 1991 [PubMed 1840704]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-ZEB1 antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX105278

GTX105278 IP Image

ZEB1 antibody immunoprecipitates ZEB1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of ZEB1 protein by 2 μg of ZEB1 antibody (GTX105278) 7.5% SDS-PAGE The immunoprecipitated ZEB1 protein was detected by ZEB1 antibody (GTX105278) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-ZEB1 antibody [N2C1], Internal used in Western Blot (WB). GTX105278

GTX105278 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [N2C1], Internal (GTX105278) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ZEB1 antibody [N2C1], Internal used in Western Blot (WB). GTX105278

GTX105278 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with ZEB1 antibody [N2C1], Internal (GTX105278) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-ZEB1 antibody [N2C1], Internal used in ChIP assay (ChIP assay). GTX105278

GTX105278 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-ZEB1 antibody. The precipitated DNA was detected by PCR with primer set targeting to LAMC2 promotor.
ChIP experiment and primer designs are based on Proc Natl Acad Sci U S A. 2011 Nov 29;108(48):19204-9.

Anti-ZEB1 antibody [N2C1], Internal used in Western Blot (WB). GTX105278

GTX105278 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [N2C1], Internal (GTX105278) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 19706487

Anti-ZEB1 antibody [N2C1], Internal used in Western Blot (WB). GTX105278

GTX105278 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody (GTX105278) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZEB1 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105278

GTX105278 ICC/IF Image

ZEB1 antibody [N2C1], Internal detects ZEB1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZEB1 protein stained by ZEB1 antibody [N2C1], Internal (GTX105278) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 μm.

REFERENCE

REVIEW

ZEB1 antibody [N2C1], Internal Cat No. GTX105278

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 07-Feb-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : H1975 cells
Amount used : 40 μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: shLuc, Lane 2-4: shZEB1
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf