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ZEB1 antibody

Orthogonal
Anti-ZEB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33589
Anti-ZEB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33589
Anti-ZEB1 antibody used in Western Blot (WB). GTX33589
Anti-ZEB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33589
Anti-ZEB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33589

Cat. No. GTX33589

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all ZEB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ZEB1 (NP_001167567.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zinc finger E-box binding homeobox 1 , AREB6 , BZP , DELTAEF1 , FECD6 , NIL2A , PPCD3 , TCF8 , ZFHEP , ZFHX1A

Cellular Localization

Nucleus

Background

This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-ZEB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33589

GTX33589 IHC-P Image

IHC-P analysis of human mammary cancer tissue using GTX33589 ZEB1 antibody.
Dilution : 1:200

Anti-ZEB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33589

GTX33589 IHC-P Image

IHC-P analysis of rat testis tissue using GTX33589 ZEB1 antibody.
Dilution : 1:200

Anti-ZEB1 antibody used in Western Blot (WB). GTX33589

GTX33589 WB Image

WB analysis of various sample lysates using GTX33589 ZEB1 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-ZEB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33589

GTX33589 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX33589 ZEB1 antibody.
Blue : DAPI

Anti-ZEB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33589

GTX33589 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX33589 ZEB1 antibody.
Dilution : 1:200

REFERENCE

Application Reference

Zhu X et al. J Orthop Surg Res 2022; 17 (1) : 423 ZEB1 regulates bone metabolism in osteoporotic rats through inducing POLDIP2 transcription.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399