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ZEB2 antibody

Anti-ZEB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129243
Anti-ZEB2 antibody used in Western Blot (WB). GTX129243
Anti-ZEB2 antibody used in Western Blot (WB). GTX129243
Anti-ZEB2 antibody used in Western Blot (WB). GTX129243
Anti-ZEB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129243
Anti-ZEB2 antibody used in Western Blot (WB). GTX129243

Cat No. GTX129243

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

136 kDa. ( Note )

Positive Control

MEF , A375 , Neuro 2A , C8D30 , Raw264.7 , C2C12 , rat brain , DDDDK-tagged ZEB2-transfected 293T

Predict Reactivity

Rat, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZEB2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

zinc finger E-box binding homeobox 2 , HSPC082 , SIP-1 , SIP1 , SMADIP1 , ZFHX1B

Cellular Localization

Nucleus

Background

The SMADIP1 gene (also known as SIP1) is a member of the delta-EF1 (ZEB1; MIM 189909)/Zfh1 family of 2-handed zinc finger/homeodomain proteins. SMADIP1 interacts with receptor-mediated, activated full-length SMADs (see MIM 605568) (Verschueren et al., 1999 [PubMed 10400677]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-ZEB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129243

GTX129243 ICC/IF Image

ZEB2 antibody detects ZEB2 protein at nucleus by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZEB2 protein stained by ZEB2 antibody (GTX129243) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-ZEB2 antibody used in Western Blot (WB). GTX129243

GTX129243 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB2 antibody (GTX129243) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ZEB2 antibody used in Western Blot (WB). GTX129243

GTX129243 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZEB2 antibody (GTX129243) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZEB2 antibody used in Western Blot (WB). GTX129243

GTX129243 WB Image

Mouse tissue extract (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB2 antibody (GTX129243) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ZEB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129243

GTX129243 IHC-P Image

ZEB2 antibody detects ZEB2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
ZEB2 stained by ZEB2 antibody (GTX129243) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ZEB2 antibody used in Western Blot (WB). GTX129243

GTX129243 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB2 antibody (GTX129243) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169