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ZEB2 antibody

Orthogonal Overexpression
Anti-ZEB2 antibody used in Western Blot (WB). GTX129243
Anti-ZEB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129243
Anti-ZEB2 antibody used in Western Blot (WB). GTX129243
Anti-ZEB2 antibody used in Western Blot (WB). GTX129243
Anti-ZEB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129243
Anti-ZEB2 antibody used in Western Blot (WB). GTX129243
Anti-ZEB2 antibody used in Western Blot (WB). GTX129243

Cat. No. GTX129243

Reference 7

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ZEB2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

136 kDa. ( Note )

Positive Control

DDDDK-tagged ZEB2-transfected 293T

Predict Reactivity

Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZEB2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885939

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zinc finger E-box binding homeobox 2 , HSPC082 , SIP-1 , SIP1 , SMADIP1 , ZFHX1B

Cellular Localization

Nucleus

Background

The SMADIP1 gene (also known as SIP1) is a member of the delta-EF1 (ZEB1; MIM 189909)/Zfh1 family of 2-handed zinc finger/homeodomain proteins. SMADIP1 interacts with receptor-mediated, activated full-length SMADs (see MIM 605568) (Verschueren et al., 1999 [PubMed 10400677]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-ZEB2 antibody used in Western Blot (WB). GTX129243

GTX129243 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB2 antibody (GTX129243) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ZEB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129243

GTX129243 IHC-P Image

ZEB2 antibody detects ZEB2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
ZEB2 stained by ZEB2 antibody (GTX129243) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ZEB2 antibody used in Western Blot (WB). GTX129243

GTX129243 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZEB2 antibody (GTX129243) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZEB2 antibody used in Western Blot (WB). GTX129243

GTX129243 WB Image

Mouse tissue extract (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB2 antibody (GTX129243) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ZEB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129243

GTX129243 ICC/IF Image

ZEB2 antibody detects ZEB2 protein at nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZEB2 stained by ZEB2 antibody (GTX129243) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-ZEB2 antibody used in Western Blot (WB). GTX129243

GTX129243 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB2 antibody (GTX129243) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZEB2 antibody used in Western Blot (WB). GTX129243

GTX129243 WB Image

Orthogonal

The data was published in the journal Cancer Med in 2020.PMID: 32277605

REFERENCE

Application Reference

Ji Y et al. Cancer Med 2020; 9 (11) : 3954-3963 Long noncoding RNA MEG3 decreases the growth of head and neck squamous cell carcinoma by regulating the expression of miR-421 and E-cadherin.
Lee JY et al. PLoS One 2020; 15 (7) : e0236876 PHRF1 promotes migration and invasion by modulating ZEB1 expression.
Application : WB
Reactivity : Human
Pei YF et al. Am J Pathol 2020; 190 (6) : 1343-1354 The Crosstalk between lncRNA-SNHG7/miRNA-181/cbx7 Modulates Malignant Character in Lung Adenocarcinoma.
Application : WB
Reactivity : Human
Pei YF et al. Am J Pathol 2019; (Epub) Methyl-CpG Binding Domain Protein 2 Inhibits the Malignant Characteristic of Lung Adenocarcinoma through the Epigenetic Modulation of 10 to 11 Translocation 1 and miR-200s.
Application : WB
Reactivity : Human
Li JM et al. Ther Adv Med Oncol 2018; 10 : 1.76E+15 Upregulation of LGALS1 is associated with oral cancer metastasis.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169