GeneTex
United States (US)

ZEB2 antibody

Overexpression
GTX129243_41563_IFA_18121410_785.jpg
GTX129243_41563_20181207_WB_B_19011519_181.jpg
GTX129243_41563_20151029_WB_Merged_18121410_805.jpg
GTX129243_41563_20151015_WB_M_embryonic fibroblast_18121410_238.jpg

Cat No. GTX129243

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

136 kDa. ( Note )

Positive Control

MEF , A375 , Neuro 2A , C8D30 , Raw264.7 , C2C12 , rat brain , DDDDK-tagged ZEB2-transfected 293T

Predict Reactivity

Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.36mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZEB2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

SIP-1 Antibody, SMADIP1 Antibody, HSPC082 Antibody, ZEB2 Antibody, zinc finger E-box binding homeobox 2 Antibody, ZFHX1B Antibody

Cellular Localization

Nucleus

Background

The SMADIP1 gene (also known as SIP1) is a member of the delta-EF1 (ZEB1; MIM 189909)/Zfh1 family of 2-handed zinc finger/homeodomain proteins. SMADIP1 interacts with receptor-mediated, activated full-length SMADs (see MIM 605568) (Verschueren et al., 1999 [PubMed 10400677]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX129243_41563_IFA_18121410_785.jpg

GTX129243 ICC/IF Image

ZEB2 antibody detects ZEB2 protein at nucleus by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZEB2 protein stained by ZEB2 antibody (GTX129243) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX129243_41563_20181207_WB_B_19011519_181.jpg

GTX129243 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB2 antibody (GTX129243) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX129243_41563_20151029_WB_Merged_18121410_805.jpg

GTX129243 WB Image

ZEB2 antibody detects ZEB2 protein by western blot analysis. Various whole cell extracts (30 µg) and rat tissue extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB2 antibody (GTX129243) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX129243_41563_20151015_WB_M_embryonic fibroblast_18121410_238.jpg

GTX129243 WB Image

ZEB2 antibody detects ZEB2 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZEB2 antibody (GTX129243) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf