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ZEB2 antibody

Anti-ZEB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85180
Anti-ZEB2 antibody used in Western Blot (WB). GTX85180
Anti-ZEB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85180
Anti-ZEB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85180
Anti-ZEB2 antibody used in Western Blot (WB). GTX85180

Cat. No. GTX85180

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all ZEB2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
ICC/IF 20 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

136 kDa. ( Note )

Positive Control

Jurkat Cell Slide(GTX25533) , EL4 Cell Lysate(GTX27183)

Product Note

ZEB2 antibody is predicted to not cross-react with ZEB1.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ZEB2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human ZEB2.The immunogen is located within the last 50 amino acids of ZEB2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10734383

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zinc finger E-box binding homeobox 2 , HSPC082 , SIP-1 , SIP1 , SMADIP1 , ZFHX1B

Cellular Localization

Nucleus

Background

ZEB2, initially identified as Smad interacting-protein 1, is normally located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Like the homologous ZEB1, ZEB2 inhibits the transcription of the E-cadherin gene and induces epithelial-mesenchymal transition, a genetic program controlling cell migration during embryonic development and wound healing, in vitro. ZEB2 can also protect cells from DNA damage-induced apoptosis, suggesting that its expression may contribute to tumor progression. Recent evidence has shown that ZEB2 is often observed in the cytoplasm in numerous cancer tissues, indicating that its localization may be regulated in normal and tumor tissues (3). Mutations in this gene are also associated with Hirschsprung disease/Mowat-Wilson syndrome.

Database

Research Area

DATA IMAGES

Anti-ZEB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85180

GTX85180 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX85180 ZEB2 antibody.
Working concentration : 20 μg/ml

Anti-ZEB2 antibody used in Western Blot (WB). GTX85180

GTX85180 WB Image

WB analysis of EL4 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX85180 ZEB2 antibody.
Working concentration : 1 μg/ml

Anti-ZEB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85180

GTX85180 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX85180 ZEB2 antibody.
Working concentration : 20 μg/ml

Anti-ZEB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85180

GTX85180 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX85180 ZEB2 antibody.
Working concentration : 20 μg/ml

Anti-ZEB2 antibody used in Western Blot (WB). GTX85180

GTX85180 WB Image

The data was published in the journal Oncotarget in 2016. PMID: 27102302

REFERENCE

Application Reference

Cui Y et al. Oncotarget 2016; 7 (23) : 34356-70 Asparaginyl endopeptidase promotes the invasion and metastasis of gastric cancer through modulating epithelial-to-mesenchymal transition and analysis of their phosphorylation signaling pathways.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399