Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZER1 antibody [C2C3], C-term (GTX106983) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX106983 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using ZER1(GTX106983) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min