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ZER1 antibody [C2C3], C-term

Anti-ZER1 antibody [C2C3], C-term used in Western Blot (WB). GTX106983
Anti-ZER1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106983
Anti-ZER1 antibody [C2C3], C-term used in Western Blot (WB). GTX106983

Cat No. GTX106983

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Positive Control

293T , A431 , H1299 , Molt-4 , Raji , mouse brain

Predict Reactivity

Rat, Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ZER1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952649

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

zyg-11 related cell cycle regulator , C9orf60 , ZYG , ZYG11BL

Background

Probably acts as target recruitment subunit in the E3 ubiquitin ligase complex ZER1-CUL2-Elongin BC.

Database

Research Area

DATA IMAGES

Anti-ZER1 antibody [C2C3], C-term used in Western Blot (WB). GTX106983

GTX106983 WB Image

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
B:H1299
7.5% SDS PAGE
GTX106983 diluted at 1:500

Anti-ZER1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106983

GTX106983 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using ZER1(GTX106983) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ZER1 antibody [C2C3], C-term used in Western Blot (WB). GTX106983

GTX106983 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX106983 diluted at 1:1000

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169