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ZER1 antibody [C2C3], C-term

Anti-ZER1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106983
Anti-ZER1 antibody [C2C3], C-term used in Western Blot (WB). GTX106983
Anti-ZER1 antibody [C2C3], C-term used in Western Blot (WB). GTX106983

Cat. No. GTX106983

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ZER1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Positive Control

A431 , H1299 , Mouse brain

Predict Reactivity

Rat, Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ZER1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952649

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zyg-11 related cell cycle regulator , C9orf60 , ZYG , ZYG11BL

Background

Probably acts as target recruitment subunit in the E3 ubiquitin ligase complex ZER1-CUL2-Elongin BC.

Database

Research Area

DATA IMAGES

Anti-ZER1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106983

GTX106983 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using ZER1(GTX106983) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ZER1 antibody [C2C3], C-term used in Western Blot (WB). GTX106983

GTX106983 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX106983 diluted at 1:1000

Anti-ZER1 antibody [C2C3], C-term used in Western Blot (WB). GTX106983

GTX106983 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZER1 antibody [C2C3], C-term (GTX106983) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

White EA et al. Proc Natl Acad Sci U S A 2012; 109 (5) : E260-7 Systematic identification of interactions between host cell proteins and E7 oncoproteins from diverse human papillomaviruses.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169