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ZFP57 antibody [GT4512]

Anti-ZFP57 antibody [GT4512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631715
Anti-ZFP57 antibody [GT4512] used in Western Blot (WB). GTX631715

Cat. No. GTX631715

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT4512

Isotype

 IgG2b

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ZFP57 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

3xFlag-human ZFP57-transfected 293T cells

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ZFP57. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888253

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ZFP57 zinc finger protein , C6orf40 , TNDM1 , ZNF698 , bA145L22 , bA145L22.2

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a zinc finger protein containing a KRAB domain. Studies in mouse suggest that this protein may function as a transcriptional repressor. Mutations in this gene have been associated with transient neonatal diabetes mellitus type 1 (TNDM1).

Database

Research Area

DATA IMAGES

Anti-ZFP57 antibody [GT4512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631715

GTX631715 ICC/IF Image

ZFP57 antibody [GT4512] detects ZFP57 protein at nucleus by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZFP57 stained by ZFP57 antibody [GT4512] (GTX631715) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-ZFP57 antibody [GT4512] used in Western Blot (WB). GTX631715

GTX631715 WB Image

ZFP57 antibody detects ZFP57 protein by western blot analysis. Non-transfected (-) and ZFP57 -transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZFP57 antibody (GTX631715) diluted by 1:5000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations