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ZFP57 antibody [GT4512]

Anti-ZFP57 antibody [GT4512] used in Western Blot (WB). GTX631715
Anti-ZFP57 antibody [GT4512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631715

Cat No. GTX631715

Host Mouse
Clonality Monoclonal
Clone Name GT4512
Isotype IgG2b
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

3xFlag-human ZFP57-transfected 293T
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ZFP57. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ZFP57 zinc finger protein,C6orf40,TNDM1,ZNF698,bA145L22,bA145L22.2

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a zinc finger protein containing a KRAB domain. Studies in mouse suggest that this protein may function as a transcriptional repressor. Mutations in this gene have been associated with transient neonatal diabetes mellitus type 1 (TNDM1).

Database

Research Area

DATA IMAGES
Anti-ZFP57 antibody [GT4512] used in Western Blot (WB). GTX631715

GTX631715 WB Image

ZFP57 antibody detects ZFP57 protein by western blot analysis. Non-transfected (-) and ZFP57 -transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZFP57 antibody (GTX631715) diluted by 1:5000.

Anti-ZFP57 antibody [GT4512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631715

GTX631715 ICC/IF Image

ZFP57 antibody [GT4512] detects ZFP57 protein at nucleus by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZFP57 stained by ZFP57 antibody [GT4512] (GTX631715) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169