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ZFX antibody

Anti-ZFX antibody used in IHC (Paraffin sections) (IHC-P). GTX31851
Anti-ZFX antibody used in IHC (Paraffin sections) (IHC-P). GTX31851
Anti-ZFX antibody used in Western Blot (WB). GTX31851

Cat No. GTX31851

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

91 kDa. ( Note )

Positive Control

Caco-2 Cell Lysate(GTX23950) , Daudi Cell Lysate(GTX23951) , Human Small Intestine Tissue Slide

Specificity/Sensitivity

Multiple isoforms of ZFX are known to exist. ZFX antibody will detect isoform 1 and 2 of ZFX.

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ZFX antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human ZFX.The immunogen is located within the last 50 amino acids of ZFX.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

zinc finger protein X-linked , ZNF926

Cellular Localization

Nucleus

Background

This gene on the X chromosome is structurally similar to a related gene on the Y chromosome. It encodes a member of the krueppel C2H2-type zinc-finger protein family. The full-length protein contains an acidic transcriptional activation domain (AD), a nuclear localization sequence (NLS) and a DNA binding domain (DBD) consisting of 13 C2H2-type zinc fingers. Studies in mouse embryonic and adult hematopoietic stem cells showed that this gene was required as a transcriptional regulator for self-renewal of both stem cell types, but it was dispensable for growth and differentiation of their progeny. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, but the full-length nature of some variants has not been determined. [provided by RefSeq, May 2010]

Database

DATA IMAGES

Anti-ZFX antibody used in IHC (Paraffin sections) (IHC-P). GTX31851

GTX31851 IHC-P Image

IHC-P analysis of human small intestine tissue using GTX31851 ZFX antibody.
Working concentration : 20 μg/ml

Anti-ZFX antibody used in IHC (Paraffin sections) (IHC-P). GTX31851

GTX31851 IHC-P Image

IHC-P analysis of human small intestine tissue using GTX31851 ZFX antibody.
Working concentration : 5 μg/ml

Anti-ZFX antibody used in Western Blot (WB). GTX31851

GTX31851 WB Image

WB analysis of Daudi cell lysate using GTX31851 ZFX antibody.
Working concentration : 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339